Optimized Multicolor Flow Cytometry Panels

 

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Human T Cell Differentiation and CCRs (27c)

 

A 27-color panel focused on comprehensive T cell subset phenotyping based on chemokine receptor expression. Panel optimization was performed using a 5-laser Cytek Aurora.

 

  • Instrument used for optimization: 5-laser Cytek® Aurora (UV, V, B, YG, R)
  • Ordering information: Please contact us to order the listed reagents through a promotional bundle. Each bundle includes ancillary reagents (e.g. Fc block, staining buffer, compensation beads) in addition to the fluorophore-conjugated antibodies needed for the experiment.
  • Product Name: Human T Cell Differentiation and CCRs (27c)

 

Marker, Fluorophore, and Clone List:

 

Product Clone    
Brilliant Violet 421™ anti-human CD197 (CCR7) G043H7 View Product
Pacific Blue™ anti-human CD28 CD28.2 View Product
Brilliant Violet 510™ anti-human CD3 OKT3 View Product
Brilliant Violet 570™ anti-human CD127 (IL-7Rα) A019D5 View Product
Brilliant Violet 605™ anti-human CD183 (CXCR3) G025H7 View Product
Brilliant Violet 650™ anti-human CD196 (CCR6) G034E3 View Product
Brilliant Violet 711™ anti-human/mouse/rat CD278 (ICOS) C398.4A View Product
Brilliant Violet 750™ anti-human CD185 (CXCR5) J252D4 View Product
Brilliant Violet 785™ anti-human CD161 HP-3G10 View Product
FITC anti-human TCR Vα7.2 3C10 View Product
Spark Blue™ 550 anti-human CD8 SK1 View Product
Spark Blue™ 574 anti-human CD45 2D1 View Product
PerCP/Cyanine5.5 anti-human CD195 (CCR5) J418F1 View Product
PE anti-human CD279 (PD-1) A17188A View Product
Spark YG™ 581 anti-human CD4 SK3 View Product
PE/Dazzle™ 594 anti-human CD95 (Fas) DX2 View Product
PE/Fire™ 640 anti-human CD38 S17015F View Product
PE/Cyanine5 anti-human CD25 M-A251 View Product
PE/Fire™ 700 anti-human TCR γ/δ B1 View Product
PE/Cyanine7 anti-human CD39 A1 View Product
PE/Fire™ 810 anti-human CD194 (CCR4) L291H4 View Product
APC anti-human CCR10 6588-5 View Product
Spark NIR™ 685 anti-human CD45RA HI100 View Product
Alexa Fluor® 700 anti-human CD19 HIB19 View Product
Alexa Fluor® 700 anti-human CD14 63D3 View Product
APC/Fire™ 750 anti-human CD27 O323 View Product
APC/Fire™ 810 anti-human HLA-DR L243 View Product
Zombie NIR™ Fixable Viability Kit Viability View Product
Human TruStain FcX™ (Fc Receptor Blocking Solution)   View Product
Cell Staining Buffer   View Product
True-Stain Monocyte Blocker™   View Product
Tandem Stabilizer   View Product
Compensation Beads   View Product

 

Representative data:

 

Human PBMCs from a healthy donor stained with the Human T Cell Differentiation and CCRs (27c) panel. Samples were acquired on a 5-laser (UV/V/B/YG/R) Cytek® Aurora using manufacturer recommended standard instrument settings.

 

Notes:

  • Users’ experimental results may vary from what is shown as representative data due to numerous factors including, but not limited to: instrument setup and other auxiliary reagents used, inherent variability in biological samples, and user technique.
  • Using a flow cytometer that is compatible with selected panel is critical for success. Prior to ordering, we urge users to check available flow cytometers and their specification to ensure it is compatible with the panel.
  • Please contact our technical services team if you have any questions or require assistance on any of the above points.
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