List of Flow Cytometers and Possible Configurations
Flow Cytometer
|
Reporter
Channel
|
Channel Emission
|
Classification Channel
|
Channel
Emission
|
Compensation
needed?
|
BD FACSCalibur™
(single laser)
|
FL2
|
575 nm
|
FL3
|
670 nm
|
Yes
|
BD FACSCalibur™ (dual laser)
|
FL2
|
575 nm
|
FL4
|
660 nm
|
No*
|
BD FACSCanto™,
BD FACSCanto™ II
|
PE
|
575 nm
|
APC
|
660 nm
|
No*
|
BD™ LSR, LSRII,
BD LSR Fortessa™
|
PE
|
575-585 nm
|
APC
|
660 nm
|
No*
|
BD FACSAria™
|
PE
|
575 nm
|
APC
|
660 nm
|
No*
|
Cytek® Aurora with YG laser
|
YG1
|
575 nm
|
R4
|
720 nm
|
No&
|
Cytek® Northern Lights and Cytek® Aurora without YG laser
|
B4
|
580 nm
|
R4
|
720 nm
|
No&
|
*Compensation is not required for the specified flow cytometers when set up properly, but is recommended for consistent results.
&Unmixing for Cytek instruments is not required.
For flow cytometers not listed here, the end-user needs to set up the machine following similar guidelines. Please refer to Setup Procedure for Other Flow Cytometers below.
The setup process typically includes the following steps. Please see the detailed setup procedure that follows, regarding your specific instrument.
1) Start up the instrument following the manufacturer’s recommendations.
2) Create a template for data acquisition using your instrument’s data acquisition software. A template is a document or worksheet with density plots that allows the user to perform machine setup and data acquisition.
3) Set up the PMT voltages of each channel to be used for data acquisition using the Setup Beads provided in the kit.
4) Determine whether compensation is needed based on the configuration of your system as shown in the table above. If compensation is needed, perform compensation using the Setup Beads provided in the kit.
|
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