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- Clone
- AN-18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IFN-gamma
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 517901 | 50 µg | 72€ | ||||
| 517902 | 500 µg | 200€ | ||||
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse IFN-γ protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ELISA Capture - Quality tested
ELISPOT, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.5 - 4.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein (Cat. No. 575309) are recomended at concentration up to 2000 pg/mL. The final standard range should be determined by the end-user.. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
ELISA Capture: The purified AN-18 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as detection antibody, and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: immunoprecipitation. -
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_10900809 (BioLegend Cat. No. 517901)
AB_10900809 (BioLegend Cat. No. 517902)
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151. - Gene ID
- 15978 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- IFN-gamma
- Specificity Alt (DOES NOT SHOW ON TDS):
- IFN-γ
- App Abbreviation (DOES NOT SHOW ON TDS):
- ELISA Capture,ELISPOT,IP
- UniProt
- View information about IFN-gamma on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All IFN-γ Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse IFN-γ | AN-18 | ELISA Capture,ELISPOT,IP |
| Ultra-LEAF™ Purified anti-mouse IFN-γ | AN-18 | ELISA Capture,ELISPOT Capture,IP |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse IFN-γ
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Ultra-LEAF™ Purified anti-mouse IFN-γ
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