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Alexa Fluor® 488 anti-NFATc1 Antibody

Pricing & Availability
Clone
7A6 (See other available formats)
Regulatory Status
RUO
Other Names
Nuclear factor of activated T-cells, cytoplasmic 1, NFAT transcription complex cytosolic component, NFAT2, NFATc
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
7A6_AF488_NFATc1_Antibody_FC_041013
Human peripheral blood lymphocytes were treated with pre-chilled 1% PFA plus 70% ethanol, then intracellularly stained with NFATc1 (clone 7A6) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
  • 7A6_AF488_NFATc1_Antibody_FC_041013
    Human peripheral blood lymphocytes were treated with pre-chilled 1% PFA plus 70% ethanol, then intracellularly stained with NFATc1 (clone 7A6) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram).
Compare all formats See Alexa Fluor® 488 spectral data
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649603 25 µg 118€
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649604 100 µg 278€
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Description

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. The protein complex consists of NFAT1, NFAT2 (NFATc1 or NFATc), NFAT3, and NFAT4. All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response. NFAT proteins are predominantly expressed in cells of the immune system but are also expressed in skeletal muscle, keratinocytes and adipocytes, regulating cell differentiation programs in these cells. In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus.

Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein of human NFATc1 amino acids 197-304.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

7A6 antibody detects endogenous human NFATC1 in Western blot with an approximate molecular weight of 100 - 120 kD (different isoforms) (Cat. No. 649602). The optimal concentration should be determined by titration for each individual assay of interest.

 

Clone 7A6 Intracellular Staining Procedure:

  1. Prepare the fix/perm solution, consisting of 1% paraformaldehyde in 70% ethanol. Chill for at least one hour at -20°C.
  2. Suspend cells in cell staining buffer and perform surface stain if necessary.
  3. Centrifuge for 5 minutes at 1500 RPM and discard supernatant.
  4. Resuspend cells in 500 µL of fix/perm solution for up to 2 x 10^6 cells and gently vortex.
  5. Incubate cells on ice for 30-60 minutes in the dark.
  6. Add 2 mL PBS and centrifuge cells for 1500 RPM for 5 minutes.
  7. Wash cells 1X with 2 mL cell staining buffer, centrifuge at 1500 RPM for 5 minutes, discard supernatant.
  8. Adjust cell concentration to about 1 x 10^7 cells/mL, add 100 µL (~ 1 x 10^6 cells) to a FACS tube that already contains the staining antibody and mix gently.
  9. Incubate 15 minutes at room temperature.
  10. Wash 2X with cell staining buffer as in step 6.
  11. Resuspend cells in cell staining buffer and analyze.
Application References

(PubMed link indicates BioLegend citation)
  1. Timmerman LA, et al. 1997. J. Immunol. 159:2735. (IF)
  2. Brandt C, et al. 2010. Cytometry A. 77:607. (FC)
  3. Fan W, et al. 2012. Arthritis Rheum. 64:3715. PubMed
Product Citations
  1. Chimote AA, et al. 2016. J Autoimmun. 86:69. PubMed
  2. Cuadrado E, et al. 2018. Immunity. 48:1046. PubMed
  3. Hoffmann U, et al. 2015. PLoS One. 10: 0132484. PubMed
  4. Nonomura K, et al. 2018. Proc Natl Acad Sci U S A. 115:12817. PubMed
  5. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  6. Guichard V, et al. 2017. Elife. 6:e27215. PubMed
  7. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  8. Morelli AE, et al. 2020. Cell Rep. 30:3448. PubMed
RRID
AB_2561822 (BioLegend Cat. No. 649603)
AB_2561822 (BioLegend Cat. No. 649604)

Antigen Details

Structure
943 amino acids with predicted molecular weight of 101 kD.
Distribution

Cytoplasm, Nucleus. NFATc1 is cytoplasmic in the phosphorylated form and nuclear after calcineurin-mediated dephosphorylation controlled activation.

Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Zhao Q, et al. 2010. Int. J. Biochem. Cell Biol. 42:576.
2. Hoey T, et al. 1995. Immunity 2:461.
3. Northrop JP, et al. 1993. J. Biol. Chem. 268:2917.
4. Hogan PG, et al. 2003. Genes Dev. 17:2205.
5. Shaw KT, et al. 1995. P. Natl. Acad. Sci. USA 92:11205.
6. Serfling E, et al. 2007. Sci. STKE 138:pe42.

Gene ID
4772 View all products for this Gene ID
UniProt
View information about NFATc1 on UniProt.org
Go To Top Version: 6    Revision Date: 06/15/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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