7 GTP cap of the mRNA. It could unwind mRNA secondary structure at the 5’ UTR region and lead to the small ribosomal subunit binding and followed sca'>
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Alexa Fluor® 594 anti-eIF4A1 Antibody

Pricing & Availability
Clone
W17130C (See other available formats)
Regulatory Status
RUO
Other Names
EIF4A1, Eukaryotic Translation Initiation Factor 4A1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17130C_A594_elF4A1_Antibody_051019.png
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl Antibody (Negative, Cat. No. 400555) or (B-D) Alexa Fluor® 594 anti EIF4A1/DDX2A Antibody (Clone W17130C) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/20. Concentrations for (A-B) is 0.4 µg/mL, (C) is 0.2 µg/mL and (D) is 0.1 µg/mL.
  • W17130C_A594_elF4A1_Antibody_051019.png
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl Antibody (Negative, Cat. No. 400555) or (B-D) Alexa Fluor® 594 anti EIF4A1/DDX2A Antibody (Clone W17130C) overnight at 4°C. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A-D) is 1/20. Concentrations for (A-B) is 0.4 µg/mL, (C) is 0.2 µg/mL and (D) is 0.1 µg/mL.
See Alexa Fluor® 594 spectral data
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666203 25 µg 128€
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666204 100 µg 296€
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Description

EIF4A1 is an ATP-dependent RNA helicase. It can form the eIF4F complex, which is involved in translation initiation by binding with the 5' m7 GTP cap of the mRNA. It could unwind mRNA secondary structure at the 5’ UTR region and lead to the small ribosomal subunit binding and followed scanning of the initiator codon.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Hamster
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human EIF4A1 Peptide (3-19 a.a.) conjugated to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 0.4 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2810694 (BioLegend Cat. No. 666203)
AB_2810694 (BioLegend Cat. No. 666204)

Antigen Details

Distribution

Cytoplasm

Function
Translation initiation
Interaction
eIF4 complex
Biology Area
Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Synthesis, Signal Transduction
Gene ID
1973 View all products for this Gene ID
UniProt
View information about EIF4A1 on UniProt.org
Go To Top Version: 1    Revision Date: 05/10/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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