Alexa Fluor® 647 anti-FOXA1 Antibody, FOXA1, 2F83

Pricing & Availability

Clone: 2F83 (See other available formats)
Regulatory Status: RUO
Other Names: Forkhead box protein A1, HNF3α, Hepatic nuclear factor 3 α
Isotype: Mouse IgG1, κ
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Product Citations: publications

MCF-7 cells were treated with True-Nuclear™ Transcription Factor Buffer Set then stained with anti-FOXA1 (clone 2F83) Alexa Fluor® 647 (filled histogram) or Mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).

See Alexa Fluor® 647 spectral data

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612003

25 µg

141€

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612004

100 µg

352€

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Description

FOXA1 is a transcriptional activator that is required for tissue-specific differentiation during embryonic development. It also functions as a coactivator with nuclear hormone receptors, including ESR1, and has been shown to play a major role in driving estrogen receptor positive breast cancers.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant human FOXA1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 5 µl per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: When using this clone for western blot, we highly recommend using lysate that has not been subjected to any freeze-thaw cycles.
Additional Product Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
RRID: AB_2801117 (BioLegend Cat. No. 612003)
AB_2801117 (BioLegend Cat. No. 612004)

Antigen Details

Structure: FOXA1 is a 472 amino acid protein with a predicted molecular weight of 49 kD.
Distribution: Nucleus/Early endoderm derived cells
Function: Transcriptional activator
Interaction: FOXA2, NKX2-1, Histones H3 and H4
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 3169 View all products for this Gene ID
UniProt: View information about FOXA1 on UniProt.org

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Other Formats

View All FOXA1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-FOXA1	2F83	WB,ICC,IHC-P
Alexa Fluor® 647 anti-FOXA1	2F83	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04/05/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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