Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2) Antibody, Glutamic Acid Decarboxylase (GAD65), N-GAD65

Pricing & Availability

Clone: N-GAD65 (See other available formats)
Regulatory Status: RUO
Other Names: Glutamate Decarboxylase 2, GAD65, Glutamate Decarboxylase 65 kD Isoform, GABA
Isotype: Mouse IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

N-GAD65_Alexa-647_Glutamic_IHC-P_040124 — IHC staining of Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm

See Alexa Fluor® 647 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

844505

25 µg

152€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

844506

100 µg

384€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

GAD gene encodes one of the several forms of glutamic acid decarboxylase, which is identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid (GABA) from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. GAD65 targets presynaptic clusters in axons and exhibits relative dendritic exclusion. GAD65 synthesizes GABA for neurotransmission and is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter (VGAT), which, as a complex, helps package GABA into vesicles for release during neurotransmission.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Peptide immunogen corresponding to amino acids 4-22 of human GAD65.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation.¹

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844503) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

This antibody demonstrates weaker reactivity to human compared to mouse GAD65/GAD2
Additional Product Notes: For immunohistochemistry (IHC), we recommend antigen retrieval with Sodium Citrate (Cat. No. 928502). Antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) produces a detectable but dimmer signal and is not recommended.

This is product is not recommended for use in the pancreas
RRID: AB_3106154 (BioLegend Cat. No. 844505)
AB_3106154 (BioLegend Cat. No. 844506)

Antigen Details

Structure: 585 amino acids with a predicted molecular weight of 65 kD.
Distribution: Cytoplasm, cytoplasmic vesicle, golgi apparatus, plasma membrane, and marker of pre-synaptic terminals.
Function: Catalyzes production of GABA from L-glutamic acid.
Interaction: Taurine and hypotaurine metabolism, transmission across chemical synapses.
Cell Type: GABAergic Neurons
Biology Area: Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References: 1. Walls A, et al. 2011. J. of Cerebral Blood Flow & Metabolism 31:494.
Gene ID: 2572 View all products for this Gene ID
UniProt: View information about Glutamic Acid Decarboxylase on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Immunohistochemistry Protocol for Paraffin-Embedded Sections

Related Products

Description	Clone	Applications

Related Pages & Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All Glutamic Acid Decarboxylase (GAD65) Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Glutamic Acid Decarboxylase (GAD65/GAD2)	N-GAD65	WB,IHC-P,IP
Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2)	N-GAD65	IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04/01/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.