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Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2) Antibody

Pricing & Availability
Clone
N-GAD65 (See other available formats)
Regulatory Status
RUO
Other Names
Glutamate Decarboxylase 2, GAD65, Glutamate Decarboxylase 65 kD Isoform, GABA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N-GAD65_Alexa-647_Glutamic_IHC-P_040124
IHC staining of Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • N-GAD65_Alexa-647_Glutamic_IHC-P_040124
    IHC staining of Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of Alexa Fluor® 647 anti-Glutamic Acid Decarboxylase (GAD65/GAD2) (clone N-GAD65). Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
See Alexa Fluor® 647 spectral data
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844505 25 µg 152€
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844506 100 µg 384€
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Description

GAD gene encodes one of the several forms of glutamic acid decarboxylase, which is identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid (GABA) from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. GAD65 targets presynaptic clusters in axons and exhibits relative dendritic exclusion. GAD65 synthesizes GABA for neurotransmission and is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter (VGAT), which, as a complex, helps package GABA into vesicles for release during neurotransmission.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide immunogen corresponding to amino acids 4-22 of human GAD65.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunoprecipitation.1

For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 844503) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

This antibody demonstrates weaker reactivity to human compared to mouse GAD65/GAD2

Additional Product Notes

For immunohistochemistry (IHC), we recommend antigen retrieval with Sodium Citrate (Cat. No. 928502). Antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) produces a detectable but dimmer signal and is not recommended.

This is product is not recommended for use in the pancreas

Application References

(PubMed link indicates BioLegend citation)
  1. Hampe CS, et al. 2001. J. Neuroimmunol. 113:63. (IP, WB)
  2. Hampe CS, et al. 2007. Clin. Exp. Immunol. 148:72.
  3. Ziegler B, et al. 1996. Acta Diabetol. 33:225.
  4. Raju R, et al. 2005. J. Immunol. 175:7755.
RRID
AB_3106154 (BioLegend Cat. No. 844505)
AB_3106154 (BioLegend Cat. No. 844506)

Antigen Details

Structure
585 amino acids with a predicted molecular weight of 65 kD.
Distribution

Cytoplasm, cytoplasmic vesicle, golgi apparatus, plasma membrane, and marker of pre-synaptic terminals.

Function
Catalyzes production of GABA from L-glutamic acid.
Interaction
Taurine and hypotaurine metabolism, transmission across chemical synapses.
Cell Type
GABAergic Neurons
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Antigen References

1. Walls A, et al. 2011. J. of Cerebral Blood Flow & Metabolism 31:494.

Gene ID
2572 View all products for this Gene ID
UniProt
View information about Glutamic Acid Decarboxylase on UniProt.org
Go To Top Version: 1    Revision Date: 04/01/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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