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Alexa Fluor® 647 anti-HMB-45 Antibody

Pricing & Availability
Clone
HMB-45 (See other available formats)
Regulatory Status
RUO
Other Names
Melanoma Marker antibody, Human Melanoma Black, Melanocyte protein PMEL, PMEL
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HMB-45_A647_HMB-45_Antibody_1_082718
IHC staining of Alexa Fluor® 647 anti-HMB-45 antibody (clone HMB-45) on formalin-fixed paraffin-embedded human melanoma tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • HMB-45_A647_HMB-45_Antibody_1_082718
    IHC staining of Alexa Fluor® 647 anti-HMB-45 antibody (clone HMB-45) on formalin-fixed paraffin-embedded human melanoma tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10X objective. Scale Bar: 100 µm
  • HMB-45_A647_HMB-45_Antibody_2_082718
    IHC staining of Alexa Fluor® 647 anti-HMB-45 antibody (clone HMB-45) on formalin-fixed paraffin-embedded human melanoma tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale Bar: 50 µm
Compare all formats See Alexa Fluor® 647 spectral data
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911505 25 µg 128€
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911506 100 µg 296€
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Description

The HMB-45 antibody reacts with an intracytoplasmic antigen expressed by a majority of melanomas (melanotic and amelanotic) and other tumors expressing melanocytic differentiation. In general, epithelioid melanoma cells display more uniform staining than more sarcomatous spindle-shaped melanoma cells. Tumor cells of epithelial, lymphoid, glial and mesenchymal origin are negative. In normal cells, the antibody reacts with junctional and blue nevus cells and with fetal and neonatal melanocytes (variable). Intradermal nevi, non-melanocytic cells and normal adult melanocytes are negative.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application References

(PubMed link indicates BioLegend citation)
  1. Shen HQ, et al. 2013. Rom. J. Morphol. Embryol. 54(3):643û7 PubMed
RRID
AB_2750177 (BioLegend Cat. No. 911505)
AB_2750177 (BioLegend Cat. No. 911506)

Antigen Details

Structure
HMB-45 (Human Melanoma Black) is a monoclonal antibody that reacts with an antigen known as Pmel 17 present in melanocytic tumors such as melanomas.
Distribution

Tissue: expressed mainly in pigment cells of the skin, hair and eyes.
Distribution: melanocytes, intracellular.

Gene ID
6490 View all products for this Gene ID
UniProt
View information about HMB-45 on UniProt.org
Go To Top Version: 1    Revision Date: 08/27/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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