- Clone
- 3E8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- High mobility group protein 1, High mobility group protein B1, Sulfoglucuronyl carbohydrate binding protein
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![3E8_A647_HMGB1_Antibody_1_012116 3E8_A647_HMGB1_Antibody_1_012116](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/3E8_A647_HMGB1_Antibody_1_012116.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml anti-HMGB1 (clone 3E8) Alexa Fluor® 647 (red) in 5% FBS for three hours, followed by Phalloidin Alexa Fluor® 488 (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
651408 | 100 µg | 306€ |
High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains. Human HMGB1 is expressed as a 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1.
HMGB1 is a widely expressed and highly abundant protein. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 also plays a significant role in extracellular signaling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis. It acts as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. In addition, activated monocytes, macrophages, and dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils. The cytokine activity of HMGB1 is restricted to the HMG B box, while the A box is associated with the helix-loop-helix domain of transcription factors. Although HMGB1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human HMGB1 with GST tag. A universal T cell epitope from a Mycobacterium tuberculosis antigen was introduced into the C-terminus of HMGB1 to increase the immunogenicity.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone 3E8 has been reported to protect mice from LPS induced sepsis1. Additional reported applications (for relevant formats) include: neutralization1. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
-
Application References
(PubMed link indicates BioLegend citation) -
- Zhou H, et al. 2009. PLos One 4:e6087. (Neut)
- Product Citations
-
- RRID
-
AB_2566261 (BioLegend Cat. No. 651408)
Antigen Details
- Structure
- 215 amino acids with predicted molecular weight of 25 kD.
- Distribution
-
Nucleus
- Function
- DNA binding protein that associates with chromatin and has the ability to bend DNA. Binds single-stranded DNA preferentially. Involved in V(D)J recombination by acting as a cofactor of the RAG complex.
- Interaction
- Component of the RAG complex composed of core components RAG1 and RAG2.
- Cell Type
- B cells
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Thomas JO and Travers AA. 2001. Trends Biochem. Sci. 26:167.
2. Müller S, et al. 2004. J. Intern. Med. 255:332.
3. Campana L, et al. 2008. Curr. Opin. Immunol. 20:518.
4. Klune JR, et al. 2008. Mol. Med. 14:476.
5. Dumitriu IE, et al. 2005. Trends Immunol. 26:381.
6. Bonaldi T, et al. 2003. EMBO J. 22:5551. - Gene ID
- 3146 View all products for this Gene ID
- UniProt
- View information about HMGB1 on UniProt.org
Other Formats
View All HMGB1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-HMGB1 | 3E8 | WB,ICC,IHC-P |
PE anti-HMGB1 | 3E8 | ICFC |
Alexa Fluor® 594 anti-HMGB1 | 3E8 | ICC |
Alexa Fluor® 488 anti-HMGB1 | 3E8 | ICC |
Alexa Fluor® 647 anti-HMGB1 | 3E8 | ICC |
Ultra-LEAF™ Purified anti-HMGB1 | 3E8 | WB |
Direct-Blot™ HRP anti-HMGB1 | 3E8 | WB |
Brilliant Violet 421™ anti-HMGB1 | 3E8 | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-HMGB1
Total cell lysate from HeLa (lane 1, 15 µg), Raw264.7 (lane ... HeLa cells were fixed with 2% PFA for ten minutes, permeabil... IHC staining using purified anti-HMGB1 (clone 3E8) on formal... -
PE anti-HMGB1
Hela cells were fixed and permeabilized with FOXP3 Fix/Perm ... -
Alexa Fluor® 594 anti-HMGB1
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ... -
Alexa Fluor® 488 anti-HMGB1
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten... -
Alexa Fluor® 647 anti-HMGB1
HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten... -
Ultra-LEAF™ Purified anti-HMGB1
Whole cell lysates (15 µg protein) from HeLa and Raw264.7 ce... -
Direct-Blot™ HRP anti-HMGB1
Western blot analysis of 15 µg cell lysates from HL-60 (High... -
Brilliant Violet 421™ anti-HMGB1
Hela cells were fixed and permeabilized with FOXP3 Fix/Perm ...
Follow Us