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Alexa Fluor® 647 anti-human CD123 Antibody

Pricing & Availability
Clone
6H6 (See other available formats)
Regulatory Status
RUO
Other Names
IL-3Rα, IL-3 Receptor alpha
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6H6_AF647_CD123_Antibody_FC_1_050713
Human peripheral blood lymphocytes were stained with CD45RO PE and CD123 (clone 6H6) Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom).
  • 6H6_AF647_CD123_Antibody_FC_1_050713
    Human peripheral blood lymphocytes were stained with CD45RO PE and CD123 (clone 6H6) Alexa Fluor® 647 (top) or mouse IgG1 Alexa Fluor® 647 isotype control (bottom).
  • 6H6_AF647_CD123_Antibody_FC_2_050713
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
306023 25 tests 123€
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306024 100 tests 249€
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Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-3Rα transfected COS cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.

Application References

(PubMed link indicates BioLegend citation)
  1. Sun Q, et al. 1996. Blood 87:83. (IP, WB)
  2. Herling M, et al. 2003. Blood 101:5007. (IHC)
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
  5. Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  6. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  7. Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations
  1. Pham TNQ, et al. 2020. Cell Reports. 29(9):2770-2782.e5.. PubMed
  2. Huang P, et al. 2020. Proc Natl Acad Sci U S A. 117:12868. PubMed
RRID
AB_2562070 (BioLegend Cat. No. 306023)
AB_2562070 (BioLegend Cat. No. 306024)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution

Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes

Function
Hematopoietic cell proliferation, differentiation
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Miyajima A, et al. 1993. Blood 82:1960.

Gene ID
3563 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org

Other Formats

View All CD123 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human CD123 6H6 FC
PE anti-human CD123 6H6 FC,SB
Purified anti-human CD123 6H6 FC,CyTOF®,IHC-F,IP,WB
PE/Cyanine5 anti-human CD123 6H6 FC
PE/Cyanine7 anti-human CD123 6H6 FC
APC anti-human CD123 6H6 FC
FITC anti-human CD123 6H6 FC
PerCP/Cyanine5.5 anti-human CD123 6H6 FC
Brilliant Violet 421™ anti-human CD123 6H6 FC
Brilliant Violet 650™ anti-human CD123 6H6 FC
Brilliant Violet 510™ anti-human CD123 6H6 FC
Alexa Fluor® 647 anti-human CD123 6H6 FC
Brilliant Violet 605™ anti-human CD123 6H6 FC
Purified anti-human CD123 (Maxpar® Ready) 6H6 FC,CyTOF®
Brilliant Violet 711™ anti-human CD123 6H6 FC
Brilliant Violet 785™ anti-human CD123 6H6 FC
PE/Dazzle™ 594 anti-human CD123 6H6 FC
Alexa Fluor® 488 anti-human CD123 6H6 FC
PE/Cyanine7 anti-human CD123 6H6 FC
TotalSeq™-A0064 anti-human CD123 6H6 PG
Alexa Fluor® 700 anti-human CD123 6H6 FC
APC/Fire™ 750 anti-human CD123 6H6 FC
Pacific Blue™ anti-human CD123 6H6 FC
TotalSeq™-C0064 anti-human CD123 6H6 PG
TotalSeq™-B0064 anti-human CD123 6H6 PG
TotalSeq™-D0064 anti-human CD123 6H6 PG
PerCP anti-human CD123 6H6 FC
GMP PE/Cyanine7 anti-human CD123 6H6 FC
APC/Fire™ 810 anti-human CD123 Antibody 6H6 FC
APC anti-human CD123 6H6 FC
PE anti-human CD123 6H6 FC
Pacific Blue™ anti-human CD123 6H6 FC
Spark Red™ 718 anti-human CD123 (Flexi-Fluor™) 6H6 FC
PerCP anti-human CD123 6H6 FC
PerCP/Cyanine5.5 anti-human CD123 6H6 FC
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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