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Alexa Fluor® 647 anti-human CD24 Antibody

Pricing & Availability
Clone
ML5 (See other available formats)
Regulatory Status
RUO
Workshop
V CD24.5
Other Names
Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1)ML5_Alx647_062107
Human peripheral blood lymphocytes stained ML5 Alexa Fluor® 647
  • 1)ML5_Alx647_062107
    Human peripheral blood lymphocytes stained ML5 Alexa Fluor® 647
  • ML5_AF647_CD24_Antibody_ICC_050319
    MDA-MB231 breast cancer cell line was stained with 10 µg/mL anti-human CD24 Alexa Fluor® 647 and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute
Compare all formats See Alexa Fluor® 647 spectral data
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311109 25 tests 100€
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311110 100 tests 212€
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Description

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
  2. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  3. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
  4. Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Product Citations
  1. Rajput S, et al. 2019. Oncotarget. 10:3667. PubMed
  2. Lazzara J 2014. Cancer Res. 74:309. PubMed
  3. Burbano YCB, et al. 2019. Rev Soc Bras Med Trop. 52:e20190101. PubMed
  4. Brisard D, et al. 2018. Oncotarget. 9:37305. PubMed
  5. Tyagi N, et al. 2016. Cancer Lett. 370: 260-267. PubMed
  6. Tecle E, et al. 2019. J Biol Chem. 294:11910. PubMed
  7. Leccia F, et al. 2012. Cytometry A. 81:960. PubMed
  8. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
  9. Sun Q, et al. 2018. J Clin Invest. 128:531. PubMed
  10. Ye S, et al. 2019. Cancer Res. 79:4729. PubMed
RRID
AB_528783 (BioLegend Cat. No. 311109)
AB_528783 (BioLegend Cat. No. 311110)

Antigen Details

Structure
GPI-linked glycoprotein, 35-45 kD
Distribution

B cells, granulocytes, epithelial cells

Function
B cell proliferation and differentiation
Ligand/Receptor
CD62P (P-Selectin)
Cell Type
B cells, Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.

Gene ID
100133941 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org
Go To Top Version: 4    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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