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Alexa Fluor® 647 anti-human/mouse Cutaneous Lymphocyte Antigen (CLA) Antibody

Pricing & Availability
Clone
HECA-452 (See other available formats)
Regulatory Status
RUO
Workshop
V S075
Other Names
Cutaneous Lymphocyte-associated Antigen (CLA)
Isotype
Rat IgM, κ
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Product Citations
publications
HECA-452_CLA_AF647_FC_061314.jpg
Human peripheral blood lymphocytes were stained with CLA (clone HECA-452) Alexa Fluor® 647 (filled histogram) or rat IgM Alexa Fluor® 647 isotype control (open histogram).
  • HECA-452_CLA_AF647_FC_061314.jpg
    Human peripheral blood lymphocytes were stained with CLA (clone HECA-452) Alexa Fluor® 647 (filled histogram) or rat IgM Alexa Fluor® 647 isotype control (open histogram).
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
321309 25 tests 81€
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321310 100 tests 215€
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Description

Cutaneous lymphocyte antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb, HECA-452. It is expressed on T cells in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells as well as on monocytes, granulocytes, and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on the majority of peripheral blood leukocytes. CLA is a ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering, and rolling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes4. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. Additional reported applications (for the relevant formats) include: blocking of lymphocyte binding to E-selectin3, and immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Duijvestijn AM, et al. 1988. Am. J. Pathol. 130:147. (IHC)
  2. Picker LJ, et al. 1991. Nature 349:796. (IHC)
  3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
  4. Borges E, et al. 1997. J. Biol. Chem. 272:28786.
  5. Ren YL, et al. 2012. Am J Clin Pathol. 138:435. PubMed
Product Citations
  1. Kuri-Cervantes L, et al. 2020. STAR Protoc. 1:100154. PubMed
  2. Colomb F, et al. 2020. Cell Reports. 32(5):107991. PubMed
RRID
AB_2563661 (BioLegend Cat. No. 321309)
AB_2563661 (BioLegend Cat. No. 321310)

Antigen Details

Structure
140 kD, carbohydrate epitope of PSGL-1
Distribution

T cells in skin, subsets of peripheral blood T cells, NK cells, B cells and dendritic cells, monocytes, granulocytes

Function
Memory cells tethering and rolling
Ligand/Receptor
E-selectin, P-selectin, L-selectin
Cell Type
B cells, Dendritic cells, Granulocytes, Monocytes, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Picker LJ, et al. 1990. Am. J. Pathol. 136:1053.
2. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
3. Fuhlbrigge RC, et al. 1997. Nature 389:978.
4. Tu L, et al. 1999. J. Exp. Med. 189:241.
5. Yoshino T, et al. 1999. Cell. Immunol. 197:39.
6. Chang SE, et al. 2003. Acta Derm-Venereol. 83:162.
7. Schakel K, et al. 2002. Immunity 17:289.
8. Fuhlbrigge RC, et al. 2002. J. Immunol. 168:5645.

Gene ID
6404 View all products for this Gene ID
UniProt
View information about CLA on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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