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Alexa Fluor® 647 anti-human SSEA-4 Antibody

Pricing & Availability
Clone
MC-813-70 (See other available formats)
Regulatory Status
RUO
Other Names
Stage-Specific Embryonic Antigen-4, SSEA4
Isotype
Mouse IgG3, κ
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Product Citations
publications
MC-813-70_Alx647_090208.jpg
NCCIT (human teratocarcinoma cell line) stained with MC-813-70 Alexa Fluor® 647
  • MC-813-70_Alx647_090208.jpg
    NCCIT (human teratocarcinoma cell line) stained with MC-813-70 Alexa Fluor® 647
Compare all formats See Alexa Fluor® 647 spectral data
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330407 25 tests 132€
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330408 100 tests 296€
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Description

The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA); may contain stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Kannagi R, et al. 1983. The EMBO J. 2:2355
Product Citations
  1. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  2. Leavens KF, et al. 2020. Stem Cell Res. 50:102112. PubMed
  3. Mitchell MW, et al. 2022. Stem Cell Res. 63:102833. PubMed
  4. Li X et al. 2019. Stem cell research. 1726:14:00 . PubMed
  5. Houweling PJ, et al. 2021. Stem Cell Research. 54:102429. PubMed
  6. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  7. Ababneh NA, et al. 2020. Stem Cell Research. 49:102000. PubMed
  8. Gill D, et al. 2022. Elife. 11:. PubMed
  9. D öpper H, et al. 2020. Stem Cell Research. 72.55486111. PubMed
  10. Nur Patria Y, et al. 2020. Stem Cell Research. 42:101689.. PubMed
  11. Chou S, et al. 2012. Proc Natl Acad Sci U S A. 109:17573. PubMed
  12. Neureiter A et al. 2018. Stem Cell Res. 33:20-24 . PubMed
  13. Schipper L et al. 2018. Stem Cell Res. 33:41-45 . PubMed
  14. Gao Y et al. 2018. Stem cell research. 28:161-164 . PubMed
  15. Ababneh NA, et al. 2021. Stem Cell Research. 54:102358. PubMed
  16. Gagne AL et al. 2018. Stem cell research. 31:157-160 . PubMed
  17. Ababneh NA, et al. 2020. Stem Cell Research. 48:101925. PubMed
  18. Bi X, et al. 2017. EBioMedicine. 10.1016/j.ebiom.2017.03.018. PubMed
  19. Simic MS, et al. 2019. Sci Adv. 5:eaaw0025. PubMed
  20. Steenpass L 2017. Stem Cell Res.. 10.1016/j.scr.2017.07.005. PubMed
  21. Yang W, et al. 2015. PLoS One. 10: 0134995. PubMed
  22. Bi Y, et al. 2020. Cell Reports. 30(11):3917-3931. PubMed
  23. Ababneh NA, et al. 2020. Stem Cell Research. 48:101967. PubMed
  24. Maguire JA et al. 2019. Stem Cell Res. 1723:21:00 . PubMed
  25. Pashos EE et al. 2017. Cell stem cell. 20(4):558-570 . PubMed
  26. Menges J, et al. 2020. Stem Cell Research. 39:101517.. PubMed
  27. Bi Y, et al. 2022. Nat Commun. 13:3147. PubMed
RRID
AB_1089200 (BioLegend Cat. No. 330407)
AB_1089200 (BioLegend Cat. No. 330408)

Antigen Details

Structure
Carbohydrate epitope on glycosphingolipids
Distribution

Human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), preimplantation embryos, and erythrocytes; mouse oocytes, early cleaved embryos

Function
Regulation of differentiation
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Kannagi R, et al. 1983. The EMBO J. 2:2355
2. Henderson JK, et al. 2002. Stem Cells. 20:329
3. Brimble SN, et al. 2007. Stem Cells. 25:54
4. Battula VL, et al. 2007. Differentiation. 75:279

Gene ID
NA
UniProt
View information about SSEA-4 on UniProt.org
Go To Top Version: 3    Revision Date: 03/26/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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