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Alexa Fluor® 647 anti-human TRA-2-49 Antibody

Pricing & Availability
Clone
TRA-2-49/6E (See other available formats)
Regulatory Status
RUO
Other Names
Liver/Bone/Kidney Alkaline Phosphatase
Isotype
Mouse IgG1, κ
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Product Citations
publications
TRA-2-49slash6E_A647_TRA-2-49_Antibody_IF_101618
NCCIT cells were fixed with methanol for ten minutes then blocked with 5% FBS for 30 minutes. The cells were intracellularly stained with 5 µg/ml anti-human TRA-2-49 (clone TRA-2-49/6E) Alexa Fluor® 647 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
  • TRA-2-49slash6E_A647_TRA-2-49_Antibody_IF_101618
    NCCIT cells were fixed with methanol for ten minutes then blocked with 5% FBS for 30 minutes. The cells were intracellularly stained with 5 µg/ml anti-human TRA-2-49 (clone TRA-2-49/6E) Alexa Fluor® 647 (red) in blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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358710 100 µg 304€
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Description

TRA-2-49, also known as Liver/Bone/Kidney Alkaline Phosphatase (ALP or AP), is a tissue non-specific isoform of alkaline phosphatase-related antigen. This antigen is highly expressed on undifferentiated human embryonal carcinoma (EC) and embryonic stem (ES) cells, and is also expressed by liver, bone, and kidney cells. It has been reported that the expression level of TRA-2-49 is decreased upon differentiation; therefore measurement of TRA-2-49 expression can be used to monitor the differentiation status of pluripotent stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonal carcinoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunohistochemistry1 and immunofluorescence1.

Application References

(PubMed link indicates BioLegend citation)
  1. Zou XY, et al. 2012. Stem Cell Res. Ther. 3:43. (ICC)
RRID
AB_2566299 (BioLegend Cat. No. 358710)

Antigen Details

Distribution

Undifferentiated embryonic stem (ES) cells, embryonal carcinoma (EC) cells, most EC cell lines, bone, liver, kidney

Cell Type
Embryonic Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Andrews PW, et al. 1996. Int. J. Cancer. 66:806.
2. Yan X, et al. 2010. Stem Cells Dev. 19:469. (ICC)
3. Zou XY, et al. 2012. Stem Cell Res. Ther. 3:43. (ICC)

Gene ID
249 View all products for this Gene ID
UniProt
View information about TRA-2-49 on UniProt.org
Go To Top Version: 1    Revision Date: 12/18/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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