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Alexa Fluor® 647 anti-MAP2 Antibody

Pricing & Availability
Clone
SMI 52 (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein 2, MAP-2
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_SMI-52_A647_MAP2_Antibody_1_122017
IHC staining of Alexa Fluor® 647 anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 20 µm.
  • 1_SMI-52_A647_MAP2_Antibody_1_122017
    IHC staining of Alexa Fluor® 647 anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 20 µm.
  • 2_SMI-52_A647_MAP2_Antibody_2_122017
    IHC staining of Alexa Fluor® 647 anti-MAP2 antibody (clone SMI 52) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 20 µm.
  • 3_4_40X_cere_2019-04-26T15-52-43235
    Formalin-fixed, 300 micron-thick mouse brain (cerebellum) section was blocked, permeabilized and stained overnight with Tubulin Beta 3 (TUBB3)(clone AA10) Alexa Fluor® 594 (green) and MAP2 (clone SMI 52) Alexa Fluor® 647 (magenta) both at 5 µg/mL, optically cleared, and analyzed on a confocal microscope. Watch the video.
Compare all formats See Alexa Fluor® 647 spectral data
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801806 25 µg 104€
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801807 100 µg 259€
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Description

Microtubules are 25nm diameter protein rods found in most kinds of eukaryote cells. They are polymerized from a dimeric subunit made of one α subunit and one β tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein τ (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against Microtubule-Associated Protein 2 (MAP2).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
3D IHC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for relevant formats) include: immunocytochemistry5,6, ELISA7, and WB1,5.

SMI 52 reacts with microtubule-associated protein 2 (MAP2). This antibody reacts with most mammalian species and with frog tissue. SMI 52 recognizes neuronal cell bodies and dendrites in tissue sections and cell cultures. In immunoblots of brain cytoskeletal preparations, SMI 52 reacts with MAP2a and b, and also with a doublet at 68 kD that may represent MAP2c.

Application References

(PubMed link indicates BioLegend citation)
  1. Shi Y, et al. 2017. Cell Death Differ. 24(1):167. (WB)
  2. Shelton MA, et al. 2015. Biol. Psychiatry. 78(6):374. (IHC) PubMed
  3. Wang X, et al. 2015. PLoS One. 10: 0145441. (IHC) PubMed
  4. Kazim SF, et al. 2014. Neuro Dis. 71:110. (IHC)
  5. Cardenas-Aguayo MC, et al. 2013. PLOS ONE. (1):e53596. (ICC, WB) PubMed
  6. Lo Furno D, et al. 2013. J. Cell. Physiol. 228:2109. (ICC)
  7. Gensel J, et al. 2009. J Neurosci. 29:3956-3968. (ELISA) PubMed
Product Citations
  1. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
RRID
AB_2721422 (BioLegend Cat. No. 801806)
AB_2721422 (BioLegend Cat. No. 801807)

Antigen Details

Structure
MAP2 is made up of two bands of an apparent molecular weight of approximately 280 kD, referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70 kD on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternative transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein τ (tau).
Distribution

Cellular distribution: Plasma membrane, cytoskeleton, nucleus, and cytosol.
Tissue distribution: Predominantly in the nervous system

Function
MAP2 encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are involved in microtubule assembly. The products of these genes are neuron-specific cytoskeletal proteins that are enriched in dendrites, which implicate a role in determining and stabilizing dentritic shape during neuron development.
Interaction
MAP2 interacts with microtubules. It has also been reported to interact with KNDC1 via KIND2.
Ligand/Receptor
MAP2 isoforms are expressed in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast τ (tau) is found predominantly in neuronal axons.
Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Antigen References
  1. Zhou Y, et al. 2015, Neuro Oncol. 17(12):1578.
  2. Loeser H, et al. 2015, Am J Nephrol. 41(3):191.
Gene ID
17756 View all products for this Gene ID 25595 View all products for this Gene ID
UniProt
View information about MAP2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/29/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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