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Alexa Fluor® 647 anti-mouse CD285 (TLR5) Antibody

Pricing & Availability
Clone
ACT5 (See other available formats)
Regulatory Status
RUO
Other Names
Toll-like receptor 5, TIL3, SLEB1, MELIOS
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ACT5_AF647_TLR5_Antibody_FC_061914.jpg
Collagenase-digested C57BL/6 mouse spleen cells were stained with CD11c Brilliant Violet 421™, CD4 FITC and TLR5 (clone ACT5) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram). Data shown was gated on the CD11c+ CD4+ population.
  • ACT5_AF647_TLR5_Antibody_FC_061914.jpg
    Collagenase-digested C57BL/6 mouse spleen cells were stained with CD11c Brilliant Violet 421™, CD4 FITC and TLR5 (clone ACT5) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram). Data shown was gated on the CD11c+ CD4+ population.
Compare all formats See Alexa Fluor® 647 spectral data
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148103 25 µg 128€
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148104 100 µg 296€
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Description

CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
TLR5-transfected Ba/F3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Shibata T, et al. Int Immunol. 24:613. (IP, FC)
  2. Huh JW, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:7072. (IP)
Product Citations
  1. Xu Y, et al. 2021. iScience. 24:103445. PubMed
RRID
AB_2563694 (BioLegend Cat. No. 148103)
AB_2563694 (BioLegend Cat. No. 148104)

Antigen Details

Structure
Type 1 transmembrane protein, 98 kD
Distribution

Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium

Interaction
Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Ligand/Receptor
Flagellin
Cell Type
Dendritic cells, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587. 
2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857. 
3. Carapau D, et al. 2013. Infect. Immun. 81:4350. 
4. Kinnebrew M, et al. 2012. Immunity. 36:276. 
5. Sebastiani G, et al. 2000. Genomics. 64:230.

Gene ID
53791 View all products for this Gene ID
UniProt
View information about CD285 on UniProt.org
Go To Top Version: 1    Revision Date: 07/07/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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