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Alexa Fluor® 647 anti-mouse Tim-4 Antibody

Pricing & Availability
Clone
RMT4-54 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein-4
Isotype
Rat IgG2a, κ
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Product Citations
publications
RMT4-54_A647_022309.jpg
Balb/c mouse peritoneal macrophages stained with RMT4-54 Alexa Fluor® 647 and CD11b FITC
  • RMT4-54_A647_022309.jpg
    Balb/c mouse peritoneal macrophages stained with RMT4-54 Alexa Fluor® 647 and CD11b FITC
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130007 25 µg 118€
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130008 100 µg 278€
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Description

Tim-4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells. Tim-4 binds to Tim-1 to promote T cell proliferation by enhancing cell division and reducing apoptosis. Tim-4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TIM4-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: in vivo induction of auto-antibody production1 and blockade of dendritic cell Tim-42.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakayama M, et al. 2009. Blood. 113:3821. (FA)
  2. Yeung MY, et al. 2013. J. Immunol. 191:4447. (Block)
Product Citations
  1. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  2. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  3. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  4. Wu Z, et al. 2023. EMBO Rep. 24:e56524. PubMed
  5. Scott CL et al. 2018. Immunity. 49(2):312-325 . PubMed
  6. De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed
  7. Troutman TD, et al. 2021. STAR Protocols. 2(1):100363. PubMed
  8. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  9. Remmerie A, et al. 2020. Immunity. 53:641. PubMed
  10. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  11. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  12. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  13. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
RRID
AB_2201842 (BioLegend Cat. No. 130007)
AB_2201842 (BioLegend Cat. No. 130008)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain
Distribution

Unlike the other Tims, Tim-4 is only expressed by antigen-presenting cells, but not by T cells.

Function
Tim-4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
Interaction
Phosphatiylserine is the ligand of Tim-4 and Tim-4 is a ligand of Tim-1.
Ligand/Receptor
Phosphatidylserine, TIM1
Cell Type
Antigen-presenting cells
Biology Area
Immunology
Antigen References

1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577
2. Miyanishi M, et al. 2007. Nature 450:435
3. Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706

Gene ID
276891 View all products for this Gene ID
UniProt
View information about Tim-4 on UniProt.org
Go To Top Version: 3    Revision Date: 09/04/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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