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Alexa Fluor® 647 anti-PARP Antibody

Pricing & Availability
Clone
5A5 (See other available formats)
Regulatory Status
RUO
Other Names
Poly (ADP-ribose) polymerase
Isotype
Mouse IgG1, κ
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Product Citations
publications
5A5_A647_PARP_Antibody_1_111417
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of Alexa Fluor® 647 anti-PARP (clone 5A5) (red) in blocking buffer overnight followed by Alexa Fluor® 594 Phalloidin (green) staining for 10 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • 5A5_A647_PARP_Antibody_1_111417
    HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of Alexa Fluor® 647 anti-PARP (clone 5A5) (red) in blocking buffer overnight followed by Alexa Fluor® 594 Phalloidin (green) staining for 10 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • 5A5_A647_PARP_Antibody_2_111417
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X‐100 for ten minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/ml of Alexa Fluor®647 anti-PARP (clone 5A5)(Red) and costained with Alexa Fluor®594 CD8a(clone C8/144B)(Green) at 4°C overnight. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective.
  • 5A5_A647_PARP_Antibody_3_111417
    Human paraffin-embedded Breast Cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X‐100 for ten minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/ml of Alexa Fluor®647 anti-PARP (clone 5A5)(Red) and costained with Alexa Fluor®488 anti-human CD326(EpCAM) (clone9C4)(Green) at 4°C overnight. The nuclei were counter stained with DAPI (blue). The image was captured with a 10X objective.
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614308 100 µg 352€
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Description

PARP (Poly (ADP-ribose) polymerase) is a 113 kD nuclear protein that can exist as a homo- or hetero-dimer. This protein acts as a molecular "nick sensor" and functions in base excision repair, poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture and DNA metabolism and participates in protein modification to enhance or repress transcription. PARP is ribosylated by PARP2 and is a target for caspase cleavage during apoptosis. PARP interacts with proteins in the base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. In addition PARP forms heterodimers with PARP2, and interacts with PARP3. The 5A5 monoclonal antibody recognizes the N-terminal region of human and mouse PARP and has been shown to be useful for Western blotting and immunofluorescence staining.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant (partial), N-terminal 2/3 sequence of PARP
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, the suggested use is 2.5 - 5.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody recognizes N-terminal short cleaved PARP after Staurosporine treatment at around 24kDa.

Application References

(PubMed link indicates BioLegend citation)
  1. Bogiatzi SI, et al. 2012. J Allergy Clin Immunol. 130:233. PubMed.
  2. Gu Y, et al. 2013. Acta Biochim Biophys Sin. PubMed
RRID
AB_2721277 (BioLegend Cat. No. 614308)

Antigen Details

Structure
PARP family, BRCT domain, homo- or hetero-dimer; 113 kD
Distribution

Nuclear

Function
Molecular "nick sensor"; base excision repair, catalyzes poly(ADP-ribosyl)ation of acceptor proteins involved in chromatin architecture, DNA metabolism; protein modification may enhance or repress transcription
Interaction
Component of a base excision repair complex containing at least XRCC1, PARP2, POLB and LIG3. Heterodimerizes with PARP2, interacts with PARP3, modifies TATA-BP, YY1, Sp1, NF-B, p53 and others
Modification
Ribosylation by PARP2
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, DNA Repair/Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Cherney B, et al. 1987. P. Natl. Acad. Sci. USA 84:8370.
2. Ikejima M, et al. 1990. J. Biol. Chem. 265:21907.
3. Ying W, et al. 2001. P. Natl. Acad. Sci. USA 98:12227.
4. Noel G, et al. 2003. BMC Cell Biol. 4:7.

Regulation
Poly(ADP-ribose) glycohydrolase removes ribose chains, allows quick, transient ribosylation of proteins
Gene ID
142 View all products for this Gene ID
UniProt
View information about PARP on UniProt.org
Go To Top Version: 2    Revision Date: 11/15/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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