Alexa Fluor 647 anti-Phosphotyrosine Antibody anti-Phosphotyrosine

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Clone: PY20 (See other available formats)
Regulatory Status: RUO
Other Names: p-Tyr
Isotype: Mouse IgG2b, κ
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PY20_Alx647_020608 — Hydrogen peroxide stimulated EL4 cells intracellularly stained with PY20 Alexa Fluor® 647

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309308

100 µg

212€

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Description

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Mouse, Rat, All Species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH-conjugated phosphotyrosine
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per 10⁶ cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

NOTE: Do not use dilution buffer containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2, Western blotting^1,2, immunocytochemistry³.
RRID: AB_528951 (BioLegend Cat. No. 309308)

Antigen Details

Distribution: Phospho-Specific
Function: Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Gene ID: 5800 View all products for this Gene ID
UniProt: View information about Phosphotyrosine on UniProt.org

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Other Formats

View All Phosphotyrosine Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-Phosphotyrosine	PY20	WB
Purified anti-Phosphotyrosine	PY20	WB,ICC,ICFC,IP
Alexa Fluor® 488 anti-Phosphotyrosine	PY20	ICFC
Alexa Fluor® 647 anti-Phosphotyrosine	PY20	ICFC
APC anti-Phosphotyrosine	PY20	ICFC
PE anti-Phosphotyrosine	PY20	ICFC
Alexa Fluor® 594 anti-Phosphotyrosine	PY20	ICC

Certificate of Analysis

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Go To Top Version: 5 Revision Date: 11/11/2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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