- Clone
- 2A4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Puromycin from streptomyces alboniger
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
381507 | 25 tests | 151€ | ||||
381508 | 100 tests | 320€ |
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, which functions as a Tyr-tRNA mimetic that enters the ribosome A site and incorporates into the nascent chain C terminus. Antibodies against Puromycin can detect puromycylated nascent chains released from ribosomes and thereby quantify protein translation. This monoclonal antibody to puromycin can be used in flow cytometry, immunofluorescence or immunoblotting to measure rates of global protein synthesis (mRNA translation) in cells incubated with Puromycin.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, All species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Puromycin from Streptomyces alboniger coupled to KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1 - 10 µg/mL of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase.
For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody. - RRID
-
AB_2927826 (BioLegend Cat. No. 381507)
AB_2927826 (BioLegend Cat. No. 381508)
Antigen Details
- Distribution
-
Cytoplasm, Ribosomes
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Biology, Protein Synthesis
- Antigen References
-
- Schmidt E, et al. 2009. Nat Methods. 6:275-7.
- Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075.
- Gene ID
- NA
- UniProt
- View information about Puromycin on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Puromycin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Puromycin | 2A4 | ICFC,ICC,WB |
PE anti-Puromycin | 2A4 | ICFC |
Alexa Fluor® 488 anti-Puromycin | 2A4 | ICFC |
Alexa Fluor® 647 anti-Puromycin | 2A4 | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-Puromycin
Human peripheral blood mononuclear cells were stimulated wit... HeLa cells were treated with Puromycin (10 µg/mL) (left) for... Whole cell extracts (15 µg total protein) prepared from HeLa... Whole cell extracts (15 µg total protein) prepared fro... -
PE anti-Puromycin
The human pro-monocytic, human myeloid leukemia cell line, U... Human peripheral blood mononuclear cells were stimulated wit... -
Alexa Fluor® 488 anti-Puromycin
The human pro-monocytic, human myeloid leukemia cell line, U... -
Alexa Fluor® 647 anti-Puromycin
The human T-lymphoblast cell line, MOLT-4, was treated with ...
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