- Clone
- MHK-49 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin light chain kappa
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MHK49_AF700_Ig_light_chain_kappa_Antibody_FC_1_021113 MHK49_AF700_Ig_light_chain_kappa_Antibody_FC_1_021113](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MHK49_AF700_Ig_light_chain_kappa_Antibody_FC_1_021113.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD19 FITC and Ig light chain κ (clone MHK-49) Alexa Fluor® 700 (top) or mouse IgG1, κ Alexa Fluor® 700 isotype control (bottom). -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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316526 | 100 tests | 278€ |
The MHK-49 antibody reacts with both soluble and membrane human immunoglobulin light chain kappa (κ). It does not react with human immunoglobulin light chain lambda (λ) or heavy chain. The MHK-49 antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Lockridge JL, et al. 2013. Biol. Blood Marrow Transplant 9:1310-22. (ELISA)
- RRID
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AB_2561821 (BioLegend Cat. No. 316526)
Antigen Details
- Structure
- Ig family
- Distribution
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B cells
- Cell Type
- B cells
- Biology Area
- Immunology
- Gene ID
- 3514 View all products for this Gene ID
- UniProt
- View information about Ig light chain kappa on UniProt.org
Related Pages & Pathways
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Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Ig light chain κ
Over night cultured human peripheral blood lymphocytes stain... -
Biotin anti-human Ig light chain κ
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human Ig light chain κ
Human peripheral blood lymphocytes stained with MHK-49 FITC -
PE anti-human Ig light chain κ
CD19+ human B-lymphocytes stained with MHL-38 (an... -
APC anti-human Ig light chain κ
Human peripheral blood mononuclear cells stained with MHK-49... -
Alexa Fluor® 488 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Alexa Fluor® 647 anti-human Ig light chain κ
Human peripheral blood lymphocytes stained with MHK-49 Alexa... -
PerCP/Cyanine5.5 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Pacific Blue™ anti-human Ig light chain κ
Human peripheral blood lymphocytes were stained with CD19 AP... -
PE/Cyanine7 anti-human Ig light chain κ
-
APC/Cyanine7 anti-human Ig light chain κ
-
Brilliant Violet 421™ anti-human Ig light chain κ
Human peripheral blood lymphocytes were stained with CD19 PE... -
Alexa Fluor® 700 anti-human Ig light chain κ
Human peripheral blood lymphocytes were stained with CD19 FI... -
Purified anti-human Ig light chain κ (Maxpar® Ready)
Human PBMCs stained with 142Nd-anti-CD19 (HIB19) and 160Gd-a... -
APC/Fire™ 750 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
TotalSeq™-A0894 anti-human Ig light chain κ
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TotalSeq™-C0894 anti-human Ig light chain κ
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TotalSeq™-B0894 anti-human Ig light chain κ
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TotalSeq™-D0894 anti-human Ig light chain κ
-
PE/Dazzle™ 594 anti-human Ig light chain κ
Overnight cultured human peripheral blood lymphocytes were s... -
Spark Red™ 718 anti-human Ig light chain κ (Flexi-Fluor™)
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PE anti-human Ig light chain κ
Typical results from overnight cultured human peripheral blo... -
FITC anti-human Ig light chain κ
Typical results from overnight cultured human peripheral blo... -
Pacific Blue™ anti-human Ig light chain κ
Typical results from overnight cultured human peripheral blo... -
APC anti-human Ig light chain κ
Typical results from overnight cultured human peripheral blo...
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