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APC anti-human CD271 (NGFR) Antibody

Pricing & Availability
Clone
ME20.4 (See other available formats)
Regulatory Status
RUO
Other Names
p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, NGFR, Nerve Growth Factor Receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
ME20dot4_APC_040810
Human neuroblastoma cell line, SK-N-MC, stained with ME20.4 APC
  • ME20dot4_APC_040810
    Human neuroblastoma cell line, SK-N-MC, stained with ME20.4 APC
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345107 25 tests 124€
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345108 100 tests 261€
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Description

CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, and NGFR, is a type I transmembrane protein with a MW of 75 kD. It is expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands (NGF, BDNF, NTF3, and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis, and to regulate cell differentiation and neurogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WM245 melanoma cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting4, immunohistochemical staining of frozen or paraffin embedded tissue sections1,2, and blocking of NGF binding1.

Application References

(PubMed link indicates BioLegend citation)
  1. Ross A, et al. 1984. P. Natl. Acad. Sci. USA 81:6681. (FC, IP, Block, IHC)
  2. Cattoretti G, et al. 1993. Blood 81:1726. (IHC)
  3. Kamke MR, et al. 2005. Hear Res. 206:89.
  4. Baker D, et al. 1989. Cancer Res. 49:4142-4146. (FC, WB)
Product Citations
  1. Torre E et al. 2018. Cell systems. 6(2):171-179 . PubMed
  2. Peng K, et al. 2020. Sci Rep. 10:18433. PubMed
  3. Torre EA, et al. 2021. Nat Genet. 53:76. PubMed
  4. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  5. Basil MC, et al. 2022. Nature. 604:120. PubMed
  6. Fahl SP, et al. 2018. J Immunol. 200:3450. PubMed
  7. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  8. Gu P, et al. 2016. Oncogene. 10.1038/onc.2016.405. PubMed
  9. Greenwood EJD et al. 2019. Cell Rep. 27(5):1579-1596 . PubMed
  10. Brun J, et al. 2015. PLoS One. 10: 0145153. PubMed
  11. Berical A, et al. 2022. Nat Commun. 13:4270. PubMed
  12. Leibel SL, et al. 2022. iScience. 25:103797. PubMed
  13. Duan X, et al. 2022. Cell Biosci. 12:60. PubMed
  14. Kumar B, et al. 2022. Sci Adv. 8:eabh3375. PubMed
  15. Portillo AL, et al. 2021. STAR Protoc. 2:100956. PubMed
  16. Stowe CL, et al. 2019. eLife. 0.333333333333333. PubMed
  17. Jiang M, et al. 2021. Developmental Cell. 56(11):1646-1660.e5. PubMed
  18. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  19. Bazot Q, et al. 2014. Nucleic Acids Res. 42:9700. PubMed
  20. Shalabi SF, et al. 2021. Nat Aging. 1:838. PubMed
  21. Suzuki S, et al. 2021. STAR Protocols. 2:100683. PubMed
  22. Loo CS, et al. 2020. Immunity. 53:143. PubMed
  23. Barbarani G, et al. 2019. Sci Rep. 9:3388. PubMed
  24. Kumar B, et al. 2018. Cancer Res. 78:5107. PubMed
  25. Hawkins FJ, et al. 2020. Cell Stem Cell. 28(1):79-95.e8. PubMed
  26. Shin B, et al. 2020. Cell Rep. 1898:30. PubMed
  27. Hu Y, et al. 2016. J Exp Med. 213: 2759 - 2772. PubMed
  28. Rodrigues Toste de Carvalho AL, et al. 2021. Nat Protoc. 16:1802. PubMed
  29. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
  30. Boshuizen J, et al. 2020. Nat Commun. 3.198611111. PubMed
RRID
AB_10639737 (BioLegend Cat. No. 345107)
AB_10639737 (BioLegend Cat. No. 345108)

Antigen Details

Structure
Type I transmembranal protein with a MW of 75 kD. The extracellular portion contains four TNFR-Cys repeats that form the NGF binding domain; the intracellular portion contains a death domain.
Distribution

Expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells, melanocytes.

Function
Apoptosis, differentiation, neurogenesis.
Interaction
TRAF2, TRAF4, TRAF6, PTPN13, RANBP9.
Ligand/Receptor
Nerve growth factor (NGF), Brain-derived neurotrophic factor (BDNF), Neurotrophin 3. (NTF3) , Neurotrophin 4 (NTF4)
Bioactivity
Apoptosis, differentiation, neurogenesis.
Cell Type
Dendritic cells, Mesenchymal cells, Mesenchymal Stem Cells, Neurons
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, Postsynaptic proteins
Antigen References

1. Friedman MJ, et al. 2009. J. Biol. Chem. 284:27944.
2. Rock JR, et al. 2009. Proc Natl Acad Sci USA. 106:12771.
3. Kidd SK, et al. 2008. Brain Res.1195:113.
4. Peters EM, et al. 2007. Am J Pathol. 171:1872.
5. Jansen P, et al. 2007. Nat Neurosci. 10:1449.

Gene ID
4804 View all products for this Gene ID
UniProt
View information about CD271 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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