APC anti-human CD40 Antibody anti-CD40

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Clone: HB14 (See other available formats)
Regulatory Status: RUO
Workshop: V CD40.5
Other Names: BP50, TNFRSF5
Isotype: Mouse IgG1, κ
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Product Citations: publications

HB14_APC_062507 — Human peripheral blood lymphocytes stained with HB14 APC

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313008

100 tests

252€

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Description

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation, partial inhibition of CD40 binding to CD40L, and prevention of B cell apoptosis.¹ Alone, or in combination with TLR ligands, clone HIB14 stimulates B cells to produce IL-10 and differentiates it into regulatory B10 (IL-10 producing B cells).⁷ The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 313019 & 313020).
RRID: AB_314971 (BioLegend Cat. No. 313008)

Antigen Details

Structure: TNFR superfamily, type I glycoprotein, 48 kD
Distribution: B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
Function: B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Ligand/Receptor: CD154 (CD40 ligand)
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
Gene ID: 958 View all products for this Gene ID
UniProt: View information about CD40 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD40 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD40	HB14	FC,Costim
FITC anti-human CD40	HB14	FC
PE anti-human CD40	HB14	FC
APC anti-human CD40	HB14	FC
PE/Cyanine7 anti-human CD40	HB14	FC
PerCP/Cyanine5.5 anti-human CD40	HB14	FC
APC/Cyanine7 anti-human CD40	HB14	FC
Ultra-LEAF™ Purified anti-human CD40	HB14	FC,Costim
APC/Fire™ 750 anti-human CD40	HB14	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

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