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APC anti-human CD41/CD61 Antibody

Pricing & Availability
Clone
A2A9/6 (See other available formats)
Regulatory Status
RUO
Other Names
gpIIb/gpIIIa
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A2A9_APC_CD41slash61_Antibody_FC_092914
Human platelets were stained with anti-human CD41/CD61 (clone A2A9/6) APC (filled histogram), or mouse IgG2a, κ APC isotype control (open histogram).
  • A2A9_APC_CD41slash61_Antibody_FC_092914
    Human platelets were stained with anti-human CD41/CD61 (clone A2A9/6) APC (filled histogram), or mouse IgG2a, κ APC isotype control (open histogram).
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359807 25 tests 100€
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359808 100 tests 233€
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Description

CD41/CD61, also known as gpIIb/IIIa, is a member of a family integrin receptors. This is a complex comprised by CD41 and CD61 through non-covalent association. CD41/CD61 is mainly expressed by platelets and megakaryocytes. The resting form of the CD41/CD61 complex is involved in platelet activation and aggregation by binding to immobilized fibrinogen. After activation, CD41/CD61 becomes a receptor for soluble fibrinogen and several other RGD-containing adhesive proteins such as von Willebrand Factor (vWF) and fibronectin. An absence or dysfunction of CD41/CD61 on the platelet surface results in an inherited bleeding disorder, called Glanzmann Thromsasthenia (GT). CD41/CD61 has been found on murine hematopoietic progenitor cells, indicating that this complex may play a role in regulating hematopoietic development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1 and blocking (aggregation)2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 359804).

A2A9/6 is recognized as having high affinity to gpIIb/IIIa. It has been shown to inhibit platelet aggregation induced by a variety of agonists and the initiation of clot formation.

Application References

(PubMed link indicates BioLegend citation)
  1. Basani RB, et al. 1996. Blood. 88:167. (IP)
  2. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. (Block)
RRID
AB_2564342 (BioLegend Cat. No. 359807)
AB_2564342 (BioLegend Cat. No. 359808)

Antigen Details

Structure
Complex of CD41 and CD61 through non-covalent association.
Distribution
Platelets and megakaryocytes.
Function
Mediates attachment to cells of diverse matrix proteins.
Ligand/Receptor
Fibrinogen, RGD-containing adhesive proteins such as vonWillebrand Factor (vWF) and fibronectin.
Cell Type
Platelets, Megakaryocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Matsumura-Takeda K, et al. 2007. Stem Cell. 25:862.
2. Corbel C, et al. 2005. 49:279.
3. Bennett JS, et al. 1983. Proc. Natl. Acad. Sci. USA. 80:2417. 
4. Clemetson KJ, et al. 1994. Curr. Opin. Hematol. 1:388.

Gene ID
3690 View all products for this Gene ID 3674 View all products for this Gene ID
UniProt
View information about CD41/CD61 on UniProt.org
Go To Top Version: 1    Revision Date: 10/01/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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