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APC anti-human/mouse Granzyme B Recombinant Antibody

Pricing & Availability
Clone
QA16A02 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA16A02_APC_GranzymeB_Antibody_1_042817
Human peripheral blood mononuclear cells were stained with CD8 Pacific Blue™, fixed and permeabilized, and then stained with anti-Granzyme B APC (clone QA16A02, top) or mouse IgG1, κ APC isotype control (bottom).
  • QA16A02_APC_GranzymeB_Antibody_1_042817
    Human peripheral blood mononuclear cells were stained with CD8 Pacific Blue™, fixed and permeabilized, and then stained with anti-Granzyme B APC (clone QA16A02, top) or mouse IgG1, κ APC isotype control (bottom).
  • QA16A02_APC_GranzymeB_Antibody_2_042817
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372203 25 tests 100€
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372204 100 tests 261€
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Liu Z, et al. 2022. Cancer Commun (Lond). 42:971. PubMed
  2. Yi M, et al. 2022. J Immunother Cancer. 10: . PubMed
  3. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  4. Xiao J, et al. 2023. J Clin Invest. 133: . PubMed
  5. Lin J, et al. 2023. Nat Commun. 14:224. PubMed
  6. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  7. Tan J, et al. 2023. Acta Pharm Sin B. 13:632. PubMed
  8. Wang YC, et al. 2023. J Exp Med. 220:. PubMed
  9. Li D, et al. 2023. Int J Oncol. 62:. PubMed
  10. Yi J, et al. 2023. Nat Commun. 14:1941. PubMed
  11. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  12. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  13. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  14. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  15. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  16. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  17. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  18. Gong Y, et al. 2020. Cell Metabolism. 33(1):51-64.e9. PubMed
  19. Wu Y, et al. 2021. Nat Commun. 12:2346. PubMed
  20. Cooper GE, et al. 2018. Front Immunol. 9:1671. PubMed
  21. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  22. Liu W, et al. 2021. Cell Death Discov. 7:136. PubMed
  23. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  24. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  25. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  26. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  27. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  28. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  29. Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
  30. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  31. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
  32. Chen YP, et al. 2020. Cell Res. 30:1024. PubMed
  33. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  34. Zhang B, et al. 2021. Nature. 599:471. PubMed
  35. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
RRID
AB_2687027 (BioLegend Cat. No. 372203)
AB_2687027 (BioLegend Cat. No. 372204)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Caspase-3
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
T cells, NK cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org
Go To Top Version: 0    Revision Date: 05/01/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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