- Clone
- W18166A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interluekin 6 receptor alpha, CD126
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
160407 | 25 µg | 96€ | ||||
160408 | 100 µg | 233€ |
CD126 (IL-6Rα) is an 80kD member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse IL-6R recombinant protein, 1-357 a.a.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone W18166A binds to IL-6 receptor alpha, inhibits IL-6/IL-6 receptor interaction and mediated phosphorylation of signal transducer and activator of transcription 3 (STAT3). This clone does not cross-react with rat.
- RRID
-
AB_2894646 (BioLegend Cat. No. 160407)
AB_2894646 (BioLegend Cat. No. 160408)
Antigen Details
- Structure
- Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
- Distribution
-
T cells and activated B cells, monocytes, hepatocytes, plasma cells
- Function
- Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
- Ligand/Receptor
- IL-6
- Cell Type
- B cells, Epithelial cells, Fibroblasts, Granulocytes, Monocytes, Plasma cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
Taga T, et al. 1997. Annu Rev Immunol. 15:797-819.
Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London.
Boulanger MJ, et al. 2003. Science. 300:2101-4
- Gene ID
- 16194 View all products for this Gene ID
- UniProt
- View information about CD126 on UniProt.org
Other Formats
View All CD126 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD126 (IL-6Rα) | W18166A | FC,Block |
APC anti-mouse CD126 (IL-6Rα) | W18166A | FC |
PE anti-mouse CD126 (IL-6Rα) | W18166A | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD126 (IL-6Rα)
C57BL/6 splenocytes were stained with CD3 FITC, CD4 PerCP/Cy... Blocking of IL-6/STAT3 signaling pathway was assessed by int... -
APC anti-mouse CD126 (IL-6Rα)
C57BL/6 splenocytes were stained with anti-mouse CD3ɛ FITC a... -
PE anti-mouse CD126 (IL-6Rα)
C57BL/6 splenocytes were stained with anti-mouse CD3 FITC an...
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