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APC anti-mouse CD184 (CXCR4) Antibody

Pricing & Availability
Clone
L276F12 (See other available formats)
Regulatory Status
RUO
Other Names
Fusin, LESTR, Sdf1r, Cmkar4, PB-CKR, PBSF/SDF-1
Isotype
Rat IgG2b, κ
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Product Citations
publications
L276F12_APC_CD184_Antibody_FC_1_111313
C57BL/6 mouse thymocytes were stained with CD4 FITC and CD184 (clone L276F12) APC (top) or rat IgG2b, κ APC isotype control (bottom).
  • L276F12_APC_CD184_Antibody_FC_1_111313
    C57BL/6 mouse thymocytes were stained with CD4 FITC and CD184 (clone L276F12) APC (top) or rat IgG2b, κ APC isotype control (bottom).
  • L276F12_APC_CD184_Antibody_FC_2_111313.jpg
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146507 25 µg 120€
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146508 100 µg 280€
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Description

CD184, also known as CXCR4, is a member of the G protein coupled receptor family that binds CXCL12 (SDF1). CXCR4 and CXCL12 play an important role in immune and inflammatory responses through the regulation of cell migration and growth. CXCR4 plays a crucial role in the pathogenesis of several autoimmune diseases such as atherosclerosis, rheumatoid arthritis, and wound healing. In addition, CXCR4 is the cofactor for fusion and entry of the T cell-tropic form of HIV-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CXCR4-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo blocking1

Application References

(PubMed link indicates BioLegend citation)
  1. Costa MJ, et al. 2018. PLoS One. 13:e0194688 (Block) PubMed
Product Citations
  1. Wu Y, et al. 2021. Immunity. 54:2595. PubMed
  2. Fu JF, et al. 2022. Neoplasia. 29:100802. PubMed
  3. Ives A, et al. 2021. FASEB J. 35:e21418. PubMed
  4. Li X, et al. 2022. Cell. 185:1709. PubMed
  5. Alghamri MS, et al. 2022. ACS Nano. 16:8729. PubMed
  6. Valadares DG, et al. 2023. iScience. 26:106163. PubMed
  7. Pioli KT, et al. 2023. iScience. 26:106223. PubMed
  8. Mulcrone PL, et al. 2023. Sci Rep. 13:8436. PubMed
  9. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  10. Feng X, et al. 2023. Nat Commun. 14:3208. PubMed
  11. Gawish R, et al. 2022. Elife. 11:. PubMed
  12. Brooks JF, et al. 2021. Cell Reports. 36(9):109645. PubMed
  13. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  14. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  15. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  16. Lin C, et al. 2022. Nat Commun. 13:6869. PubMed
  17. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  18. Kinoshita M, et al. 2020. Cell Stem Cell. . PubMed
  19. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
  20. Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed
  21. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  22. Tanegashima K et al. 2017. EBioMedicine. 24:247-256 . PubMed
RRID
AB_2562785 (BioLegend Cat. No. 146507)
AB_2562785 (BioLegend Cat. No. 146508)

Antigen Details

Structure
G-protein-coupled seven transmembrane receptor, 39.7 kD
Distribution

T lymphocytes, monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, vascular smooth muscle cells (VSMCs)

Function
Development of cardiovascular and central nervous system, hematopoiesis, and colonization of BM by fetal liver-derived hematopoietic stem cells (HSCs) during embryogenesis, homing and egress of CD34+ CXCR4+ progenitor cells from bone marrow, and their migration into peripheral tissues. Critical role in homing of cancer cells to specific metastatic sites. Possible role in the ubiquitin proteosome system.
Interaction
Robo -1
Ligand/Receptor
CXCL12 (SDF-1)
Cell Type
Macrophages, Mast cells, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Ubiquitin/Protein Degradation
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kucia M, et al. 2005. Stem Cells 23:879.
2. Muller A, et al. 2001. Nature 410:50.
3. Saini V, et al. 2010. J. Biol. Chem. 285:15566.
4. Prasad A, et al. 2007. J. Leuko. Biol. 82:465. 
5. De Klerck B, et al. 2005. Arthritis Res. Ther. 7:R1208.
6. Rueda P, et al. 2008. PLoS One 3:e2543. 
7. Feng Y, et al. 1996. Science 272:872.

Gene ID
12767 View all products for this Gene ID
UniProt
View information about CD184 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/15/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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