APC anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
X54-5/7.1 (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Mouse IgG1, κ
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Product Citations
publications
X54-57.1_1_APC_1_052912
C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD64 (clone X54-5/7.1) APC (top) or mouse IgG1, κ APC isotype control (bottom). Data shown was gated on the myeloid population.
  • X54-57.1_1_APC_1_052912
    C57BL/6 mouse bone marrow cells were stained with CD11b PE and CD64 (clone X54-5/7.1) APC (top) or mouse IgG1, κ APC isotype control (bottom). Data shown was gated on the myeloid population.
  • X54-57.1_1_APC_2_052912
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139305 25 µg 86€
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139306 100 µg 214€
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
BALB/c mouse FcγRI-human IgG Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. Additional reported application (for relevant formats) include: immunoprecipitation1, and spatial biology (IBEX)5,6. Clone X54-5/7.1 is not found to be useful for Western blots1.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan PS, et al. 2003. J. Immunol. 170:2549. (IP)
  2. Ingersoll MA, et al. 2010. Blood 115:e10. (FC)
  3. Ozeri E, et al. 2012. J. Immunol. 189:146. PubMed
  4. Richardson ML, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  2. Chen R, et al. 2023. Acta Pharm Sin B. 13:128. PubMed
  3. Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed
  4. Sun JY, et al. 2023. Sci Adv. 9:eade4110. PubMed
  5. Coelho DR, et al. 2023. iScience. 26:106442. PubMed
  6. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  7. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  8. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  9. Choi H, et al. 2023. J Nanobiotechnology. 21:5. PubMed
  10. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  11. Bhattacharyya A, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L495. PubMed
  12. Jameson VJ, et al. 2022. Cytometry A. 101:922. PubMed
  13. Yu‐Han Chang et al. 2017. Immunity. 47(5):943-958 . PubMed
  14. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  15. Wang KC, et al. 2018. Immunohorizons. 2:407. PubMed
  16. Kaur K, et al. 2021. Cell Rep. 37:110178. PubMed
  17. de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed
  18. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  19. Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed
  20. Rothchild AC, et al. 2020. Bio Protoc. 10:00. PubMed
  21. Galle E, et al. 2022. Genome Biol. 23:207. PubMed
  22. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  23. Maisonneuve C, et al. 2021. Cell Reports. 34(4):108677. PubMed
  24. Qi X, et al. 2019. Nat Commun. 10:2141. PubMed
  25. Chan YH, et al. 2019. EMBO Mol Med. 11:e10092. PubMed
  26. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
  27. Marangoni F, et al. 2021. Cell. . PubMed
  28. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
  29. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  30. Qayum A, et al. 2016. J Immunol. 196: 4457 - 4467. PubMed
  31. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  32. Zhang S, et al. 2019. Mol Med Rep. 19:5377. PubMed
  33. Yang J, et al. 2022. Commun Biol. 5:124. PubMed
  34. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  35. Lagares D, et al. 2017. Sci Transl Med. 9:eaal3765. PubMed
  36. Holly Bachus et al. 2018. Immunity. 50(1):225-240 . PubMed
  37. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  38. Omori S, et al. 2021. Cell Reports. 34(6):108734. PubMed
  39. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  40. Zhou Q, et al. 2014. J Immunol. 193:496. PubMed
  41. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  42. Martin T, et al. 2018. Arch Toxicol. 92:323. PubMed
  43. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  44. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  45. Steuerman Y et al. 2018. Cell systems. 6(6):679-691 . PubMed
  46. Qian J, et al. 2021. Int J Mol Sci. 22:. PubMed
  47. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  48. Meliopoulos V, et al. 2016. PLoS Pathog. 12: 1005804. PubMed
  49. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  50. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  51. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  52. Spix B, et al. 2022. Nat Commun. 13:318. PubMed
  53. Andriessen EMMA, et al. 2021. EMBO Mol Med. 13:e11754. PubMed
  54. Terhorst D, et al. 2015. J Immunol. 195: 4953 - 4961. PubMed
  55. Clark JT, et al. 2021. eLife. 10:00. PubMed
  56. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
  57. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  58. Camara A, et al. 2019. Immunity. 50:1467. PubMed
  59. Penke LR, et al. 2020. Life Sci Alliance. 3:. PubMed
  60. Morikawa M, et al. 2016. PLoS One. 11:e0163607. PubMed
  61. Sencio V, et al. 2020. Cell Reports. 30(9):2934-2947. PubMed
  62. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  63. Ntokou A, et al. 2021. JCI Insight. 6:. PubMed
  64. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  65. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  66. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  67. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  68. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  69. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  70. Montalban-Arques A, et al. 2021. Cell Host Microbe. :. PubMed
  71. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  72. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  73. Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed
  74. Gong P, et al. 2022. Cell Death Discov. 8:466. PubMed
  75. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
  76. Molgora M, et al. 2020. Cell. 182:886. PubMed
  77. Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed
  78. Hildreth AD, et al. 2020. STAR Protoc. 1:100113. PubMed
  79. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  80. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  81. Rivera CA, et al. 2022. Immunity. 55:129. PubMed
  82. Boddupalli CS, et al. 2022. Elife. 11:. PubMed
  83. Joshi JC, et al. 2020. Cell Rep. 30:4096. PubMed
  84. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  85. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  86. Deets KA, et al. 2021. Elife. 10:. PubMed
  87. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  88. Huang WC, et al. 2019. Int J Hyperthermia. 36:1255. PubMed
  89. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
RRID
AB_11219391 (BioLegend Cat. No. 139305)
AB_11219391 (BioLegend Cat. No. 139306)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Cell Type
Dendritic cells, Macrophages, Mast cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org
Go To Top Version: 2    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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