APC anti-mouse IL-27 p28 Antibody anti-IL-27

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Clone: MM27-7B1 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-27 p28, IL-27-A, IL-27 subunit alpha
Isotype: Mouse IgG2a
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MM27-7B1_APC_1_082710 — Thioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ for 2 hours, followed by overnight LPS-stimulation, then intracellularly stained with CD107b (M3/84) PE and IL-27 (MM27-7B1) APC

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516906

100 µg

271€

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Description

IL-27 is a heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 P35-related protein). It is a member of the IL-6/IL-12 cytokine family and mainly produced by antigen-presenting cells, including macrophages and dendritic cells. IL-27 acts on T cells and NK cells. It has been reported that IL-27 drives rapid clonal expansion of naïve CD4⁺ T cells, and promotes Th1 polarization and IFN-γ production in synergy with IL-12. The IL-27-induced Th1 differentiation was mediated by rapid and marked upregulation of ICAM-1/LFA-1 interaction in a STAT1-dependent manner. IL-27 exhibits anti-inflammatory function by enhancing Th1 cell differentiation, a potent antitumor activity, through CD8⁺ T cell and NK cell activation. It also plays a potential therapeutic role in autoimmune disease by inhibiting Th-17 development. IL-27 mediates its biological effects through its receptor, WSX-1/T cell cytokine receptor (TCCR), which is homologous to the IL-12Rβ2 subunit. Protein gp130 serves as a functional signal-transducing molecule for IL-27.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse IL-27-OVA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone MM27-7B1 specifically recognizes the p28 subunit of IL-27.¹We confirmed clone MM27-7B1 cross-reactivity to recombinant human IL-27 in-house using an immunoassay.

ELISA Capture: To measure mouse IL-27, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated B02P6E6 antibody (Cat. No. 525904) as the detection antibody. Recombinant Mouse IL-27 (ELISA Std.) (Cat. No. 577409) can be used as the protein standard.
Flow Cytometry: The fluorochrome-labeled MM27-7B1 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.
Note: For measuring mouse IL-27 in serum, plasma or cell culture supernatant, LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit with Pre-coated Plates (Cat. No. 438707 & 438708) are specially developed and recommended.
RRID: AB_10642964 (BioLegend Cat. No. 516906)

Antigen Details

Structure: A heterodimeric cytokine consisting of EBV-induced gene-3 (EBI3, an IL-12 p40-related protein) and p28 (a newly discovered IL-12 p35-related protein).
Distribution: Produced by antigen-presenting cells, including macrophages and dendritic cells.
Function: Drives rapid clonal expansion of naïve CD4⁺ T cells, promotes Th1 polarization and IFN-γ production; plays a role in the anti-inflammatory and antitumor reponses, and has potential in autoimmune disease therapy.
Interaction: T cells and NK cells.
Ligand/Receptor: IL-27R (TCCR/WSX-1).
Cell Type: Antigen-presenting cells, Dendritic cells, Macrophages
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Salcedo R, et al. 2004. J. Immunol. 173:7170.
2. Owaki T, et al. 2005. J. Immunol. 175:2191.
3. Pflanz S, et al. 2002. Immunity 16:779.
4. Diveu C, et al. 2009. J. Immunol. 182:5748.
5. Morishima N, et al. 2005. J. Immunol. 175:1686.
6. Liu J, et al. 2007. J. Exp. Med. 204:141.
Gene ID: 246779 View all products for this Gene ID
UniProt: View information about IL-27 on UniProt.org

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Other Formats

View All IL-27 Reagents Request Custom Conjugation

Description	Clone	Applications
Alexa Fluor® 647 anti-mouse IL-27 p28	MM27-7B1	ICFC
APC anti-mouse IL-27 p28	MM27-7B1	ICFC
PE anti-mouse IL-27 p28	MM27-7B1	ICFC
PE/Cyanine7 anti-mouse IL-27 p28	MM27-7B1	ICFC
Ultra-LEAF™ Purified anti-mouse IL-27 p28	MM27-7B1	ELISA Capture,Neut
Purified anti-mouse IL-27 p28	MM27-7B1	ELISA Capture

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 09/22/2016

For Research Use Only. Not for diagnostic or therapeutic use.

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