APC anti-mouse Ly49H Antibody Ly49H, 3D10

Pricing & Availability

Clone: 3D10 (See other available formats)
Regulatory Status: RUO
Other Names: Klra8, Cmv1
Isotype: Mouse IgG1, κ
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Product Citations: publications

3D10_APC_Ly49H _Antibody_100318 — C57BL/6 mouse splenocytes were stained with CD49b (clone DX5) PE and Ly49H (clone 3D10) APC (left) or Mouse IgG1, κ APC Isotype Control (right).

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144711

25 µg

91€

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144712

100 µg

204€

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Description

Ly49H, also known as Cmv1 and Klra8, is a 28.3 kD protein. It is expressed on NK cells (but not on NKT cells) from C57BL/6 mice (but not Balb/c or DBA/2 mice). Expression of Ly49H has been associated with resistance to MCMV infection in C57BL/6 mice. Modulation of Ly49H expression by its antibody made MCMV-resistant C57BL/6 mice susceptible to infection. Unlike Ly49A, Ly49H lacks the ITIM (immunoreceptor tyrosine-based inhibitory motif) but has ITAM (immunoreceptor tyrosine-based activation motif), which is associated with DAP-12 (KARAP, killer cell activating motif).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: C1498-Ly49A/H transfectant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats of this clone) include: immunoprecipation¹, and in vitro² and in vivo³ blocking activity. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 144717 & 144718).
RRID: AB_2783111 (BioLegend Cat. No. 144711)
AB_2783111 (BioLegend Cat. No. 144712)

Antigen Details

Structure: 28.3 kD
Distribution: NK cells but not NKT cells
Function: Interacts with m157, a cytomegalovirus-encoded protein
Ligand/Receptor: m157
Cell Type: NK cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Gosselin P, et al. 1999. J. Leuko. Bio. 66:165.
2. Lee SH, et al. 2001. Nat. Genet. 28:42.
3. Daniels KA, et al. 2001. J. Exp. Med. 194:29.
Gene ID: 16639 View all products for this Gene ID
UniProt: View information about Ly49H on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All Ly49H Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse Ly49H	3D10	FC
Purified anti-mouse Ly49H	3D10	FC,IP,Block
PE anti-mouse Ly49H	3D10	FC
Alexa Fluor® 647 anti-mouse Ly49H	3D10	FC
PE/Cyanine7 anti-mouse Ly49H	3D10	FC
APC anti-mouse Ly49H	3D10	FC
TotalSeq™-A0839 anti-mouse Ly49H	3D10	PG
Ultra-LEAF™ Purified anti-mouse Ly49H	3D10	FC,IP,Block
TotalSeq™-C0839 anti-mouse Ly49H	3D10	PG
TotalSeq™-B0839 anti-mouse Ly49H	3D10	PG

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 10/04/2018

For Research Use Only. Not for diagnostic or therapeutic use.

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