APC anti-rat CD4 Antibody anti-CD4

Pricing & Availability

Clone: W3/25 (See other available formats)
Regulatory Status: RUO
Other Names: T4, L3T4, W3/25
Isotype: Mouse IgG1, κ
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Product Citations: publications

W3slash25_APC_032808 — LOU rat splenocytes stained with W3/25 APC

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201509

50 µg

143€

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Description

CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Rat thymocyte membrane glycoproteins
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections^1,2, inhibition of IL-2 production³, inhibition of MBP-induced T cell activation in EAE transfer model³.
RRID: AB_313935 (BioLegend Cat. No. 201509)

Antigen Details

Structure: Ig superfamily, 55 kD
Distribution: Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)
Function: T cell co-receptor, T cell activation
Ligand/Receptor: MHC class II molecule
Cell Type: Macrophages, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.
Gene ID: 24932 View all products for this Gene ID
UniProt: View information about CD4 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

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Description	Clone	Applications
Purified anti-rat CD4	W3/25	FC,IHC-F
FITC anti-rat CD4	W3/25	FC
PE anti-rat CD4	W3/25	FC
APC anti-rat CD4	W3/25	FC
Alexa Fluor® 488 anti-rat CD4	W3/25	FC,IHC-F
APC/Cyanine7 anti-rat CD4	W3/25	FC
PE/Cyanine7 anti-rat CD4	W3/25	FC
PerCP/Cyanine5.5 anti-rat CD4	W3/25	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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