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APC/Cyanine7 anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Regulatory Status
RUO
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM48-1_APCCy7_080412
C57BL/6 mouse splenocytes were stained with CD48 (clone HM48-1) APC/Cyanine7 (filled histogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
  • HM48-1_APCCy7_080412
    C57BL/6 mouse splenocytes were stained with CD48 (clone HM48-1) APC/Cyanine7 (filled histogram) or Armenian hamster IgG APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103431 25 µg 104€
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103432 100 µg 278€
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Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Hu R, et al. 2023. Cell Death Discov. 9:50. PubMed
  2. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  3. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  4. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  5. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
  6. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  7. Naka K, et al. 2015. Nat Commun. 6: 8039. PubMed
  8. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  9. Naka K, et al. 2020. Nat Commun. 3.709027778. PubMed
  10. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  11. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  12. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
  13. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  14. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  15. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  16. De Koninck M, et al. 2020. Cell Reports. 32(6):108014. PubMed
  17. Simon M, et al. 2019. Cell Metab. 29:871. PubMed
  18. Yoshida K, et al. 2022. Sci Rep. 12:17276. PubMed
  19. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  20. Morales Torres C, et al. 2020. Nat Commun. 11:1792. PubMed
  21. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  22. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
  23. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  24. Giannini S, et al. 2020. Nat Commun. 0.705555556. PubMed
  25. Brandi P, et al. 2022. Cell Rep. 38:110184. PubMed
  26. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  27. Rebekka K Schneider et al. 2017. Cell stem cell. 20(6):785-800 . PubMed
  28. Passaro D et al. 2017. Cancer cell. 32(3):324-341 . PubMed
  29. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  30. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  31. Yang T, et al. 2022. Nat Commun. 13:4464. PubMed
  32. Mistry JJ, et al. 2021. Nat Commun. 12:7130. PubMed
  33. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  34. Young IC, et al. 2021. Cell Rep. 37:110036. PubMed
  35. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  36. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  37. Lv K, et al. 2021. Cell Stem Cell. 28(7):1275-1290.e9. PubMed
  38. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
RRID
AB_2561462 (BioLegend Cat. No. 103431)
AB_2561462 (BioLegend Cat. No. 103432)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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