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APC/Cyanine7 Streptavidin

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Regulatory Status
RUO
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Product Citations
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APCCyanine7_Streptavidin_Antibody_042123
Human peripheral blood lymphocytes were stained with APC/Cyanine7 streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (APC/Cyanine7) Streptavidin (filled histogram).
  • APCCyanine7_Streptavidin_Antibody_042123
    Human peripheral blood lymphocytes were stained with APC/Cyanine7 streptavidin alone (open histogram) or Biotin anti-human CD3 (clone UCHT1) followed by (APC/Cyanine7) Streptavidin (filled histogram).
See APC/Cyanine7 spectral data
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405208 100 µg 165€
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Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC/Cyanine7 is useful for detecting biotinylated antibodies. The excitation of APC/Cyanine7 by 600-635 nm laser light induces a fluorescence emission maximum of 780 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin APC/Cyanine7 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this Streptavidin APC/Cyanine7 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Cyanine7 conjugation. For immunofluorescent staining, the recommended use of this reagent is =0.06 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Streptavidin-Allophycocyanin/Cyanine7 (APC/Cyanine7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC/Cyanine7 is 350 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
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Go To Top Version: 5    Revision Date: 04/21/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

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