APC/Fire 750 anti-human CD3 Antibody anti-CD3

Pricing & Availability

Clone: SK7 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ
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Product Citations: publications

SK7_APCFire750_CD3_Antibody_1_030816 — Human peripheral blood lymphocytes were stained with CD19 PE and CD3 (clone SK7) APC/Fire™ 750 (top) or mouse IgG1, κ APC/Fire™ 750 isotype control (bottom).

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344839

25 tests

120€

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344840

100 tests

254€

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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^4,5,8, immunofluorescent staining⁶, and Western blotting³.

Application References (PubMed link indicates BioLegend citation)

Kan EA, et al. 1983. J. Immunol. 131:536.
Wood GS, et al. 1985. Am. J. Pathol. 120:371.
Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
Carbone A, et al. 1999. Blood 93:2319. (IHC)
Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
Rutjens E, et al. 2007. J. Immunol. 178:1702.
Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

Product Citations

Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
Gfeller D, et al. 2023. Cell Syst. 14:72. PubMed
Mashayekhi M, et al. 2022. Prostaglandins Other Lipid Mediat. 158:106604. PubMed
Ferguson ID, et al. 2022. Nat Commun. 13:4121. PubMed
Harari A, et al. 2021. Cancers (Basel). 13:. PubMed
Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed
Seamon K, et al. 2020. Front Immunol. 11:396. PubMed
Soldi R, et al. 2020. PLoS One. 15:e0235705. PubMed
Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
Herrera FG, et al. 2019. Int J Radiat Oncol Biol Phys. 103:320. PubMed
Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
Graciotti M, et al. 2020. Vaccines (Basel). 8:00. PubMed
Warthan MD, et al. 2018. Biol Reprod. 98:309. PubMed
Kasatskaya SA, et al. 2020. Elife. :9. PubMed

RRID

AB_2572114 (BioLegend Cat. No. 344839)
AB_2572114 (BioLegend Cat. No. 344840)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, during thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

Reviews

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Cell Surface Flow Cytometry Staining Protocol

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Certificate of Analysis

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Go To Top Version: 1 Revision Date: 03/15/2016

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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