APC/Cyanine7 anti-mouse CD335 (NKp46) Antibody, CD335, 29A1.4

Pricing & Availability

Clone: 29A1.4 (See other available formats)
Regulatory Status: RUO
Other Names: NKp46, NCR1
Isotype: Rat IgG2a, κ
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Product Citations: publications

29A1-4_APCCyanine7_CD335_Antibody_070720 — C57BL/6 mouse splenocytes were stained with NK1.1 Alexa Fluor® 488 and CD335 (NKp46) (clone 29A1.4) APC/Cyanine7 (left) or rat IgG2a, κ APC/Cyanine7 isotype control (right).

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137645

25 µg

128€

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137646

100 µg

292€

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Description

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains. It's expression is restricted to NK cells and a subset of NKT cells; it's not expressed in CD1d-restricted NKT cells. CD335 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^1,2 and in vitro activation of NK cells¹.
RRID: AB_2876479 (BioLegend Cat. No. 137645)
AB_2876479 (BioLegend Cat. No. 137646)

Antigen Details

Structure: Single-pass type I membrane protein, 46 kD; belongs to the natural cytotoxicity receptor (NCR) family; contains 2 Ig-like (immunoglobulin-like) domains
Distribution: Mature and immature NK cells, subset of NKT cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand/Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.
Gene ID: 17086 View all products for this Gene ID
UniProt: View information about CD335 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD335 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 510™ anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 711™ anti-mouse CD335 (NKp46)	29A1.4	FC
PE anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Cyanine7 anti-mouse CD335 (NKp46)	29A1.4	FC
Purified anti-mouse CD335 (NKp46)	29A1.4	FC,IHC-F
FITC anti-mouse CD335 (NKp46)	29A1.4	FC
APC anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Cyanine5.5 anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 421™ anti-mouse CD335 (NKp46)	29A1.4	FC
Biotin anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 605™ anti-mouse CD335 (NKp46)	29A1.4	FC
Purified anti-mouse CD335 (NKp46) (Maxpar® Ready)	29A1.4	FC,CyTOF®
Alexa Fluor® 647 anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Dazzle™ 594 anti-mouse CD335 (NKp46)	29A1.4	FC
APC/Fire™ 750 anti-mouse CD335 (NKp46)	29A1.4	FC
Brilliant Violet 650™ anti-mouse CD335 (NKp46)	29A1.4	FC
TotalSeq™-A0184 anti-mouse CD335 (NKp46)	29A1.4	PG
Brilliant Violet 785™ anti-mouse CD335 (NKp46)	29A1.4	FC
Ultra-LEAF™ Purified anti-mouse CD335 (NKp46)	29A1.4	FC,IHC-F
TotalSeq™-B0184 anti-mouse CD335 (NKp46)	29A1.4	PG
TotalSeq™-C0184 anti-mouse CD335 (NKp46)	29A1.4	PG
APC/Cyanine7 anti-mouse CD335 (NKp46)	29A1.4	FC
PE/Cyanine5 anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Fire™ 806 anti-mouse CD335 (NKp46)	29A1.4	FC
PerCP/Fire™ 780 anti-mouse CD335 (NKp46)	29A1.4	FC
Spark Red™ 718 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC
Spark Blue™ 574 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC
Spark Blue™ 550 anti-mouse CD335 (NKp46) (Flexi-Fluor™)	29A1.4	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 07/07/2020

For Research Use Only. Not for diagnostic or therapeutic use.

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