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APC/Fire™ 750 anti-human CD162 Antibody

Pricing & Availability
Clone
KPL-1 (See other available formats)
Regulatory Status
RUO
Other Names
PSGL-1, p-selectin glycoprotein ligand-1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
KPL-1_APCFire750_CD162_Antibody_091718
Human peripheral lymphocytes were stained with CD3 PE and CD162 (clone KPL-1) APC/Fire™ 750 (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right) in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426103).
  • KPL-1_APCFire750_CD162_Antibody_091718
    Human peripheral lymphocytes were stained with CD3 PE and CD162 (clone KPL-1) APC/Fire™ 750 (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right) in the presence of True-Stain Monocyte Blocker™ (Cat. No. 426103).
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328813 25 tests 132€
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328814 100 tests 244€
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Description

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kD, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PSGL-1 transfected murine 300.19 pre B-cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone KPL-1 is reported to recognize the tyrosine sulfation consensus motif of PSGL-11. Additional reported applications (for the relevant formats) include: Western Blot1, immunoprecipitation2, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin embedded tissue sections1, blocks the recognition of PSGL-1 with P- and L-selectin1.

Application References

(PubMed link indicates BioLegend citation)
  1. Snapp KR, et al. 1998. Blood 91:154.
  2. Snapp KR, et al. 1998. J. Cell Biol. 142:263.
  3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  4. Miyamura K, et al. 2011. J. Gen. Virol. 92:287. PubMed
  5. Cheng Q, et al. 2012. Lupus. 21:632. PubMed.
Product Citations
  1. Weigert A, et al. 2022. Nat Commun. 13:6078. PubMed
RRID
AB_2750486 (BioLegend Cat. No. 328813)
AB_2750486 (BioLegend Cat. No. 328814)

Antigen Details

Distribution

Neutrophils, monocytes, and most lymphocytes

Function
Mediates the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation.
Ligand/Receptor
CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin)
Cell Type
Lymphocytes, Monocytes, Neutrophils
Biology Area
Cell Adhesion, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Snapp KR, et al. 1998. Blood 91:154.

Gene ID
6404 View all products for this Gene ID
UniProt
View information about CD162 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/18/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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