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APC/Fire™ 810 anti-mouse P2RY12 Antibody

Pricing & Availability
Clone
S16007D (See other available formats)
Regulatory Status
RUO
Other Names
P2Y12, ADPG-R, BDPLT8, HORK3, P2T(AC), P2Y(12)R, P2Y(AC), P2Y(ADP), P2Y(cyc), P2Y12P2Y12 platelet ADP receptor, purinergic receptor P2RY12, purinergic receptor P2Y, G-protein coupled, 12
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
S16007D_APCFire810_P2RY12_Antibody_111521
C57BL/6 mouse microglia were enriched from collagenase-digested brain using a Percoll® gradient. Cells were then stained with anti-mouse CX3CR1 FITC and anti-mouse P2RY12 (clone S16007D) APC/Fire™ 810 (left) or rat IgG2b, κ APC/Fire™ 810 isotype control (right).
  • S16007D_APCFire810_P2RY12_Antibody_111521
    C57BL/6 mouse microglia were enriched from collagenase-digested brain using a Percoll® gradient. Cells were then stained with anti-mouse CX3CR1 FITC and anti-mouse P2RY12 (clone S16007D) APC/Fire™ 810 (left) or rat IgG2b, κ APC/Fire™ 810 isotype control (right).
Compare all formats See APC/Fire™ 810 spectral data
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848013 25 µg 165€
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Description

Purinergic Receptor P2Y (P2RY12) is a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. P2RY12 is not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation. P2RY12 is a highly selective marker for microglia that specifically distinguishes these cells from other myeloid cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse P2RY12.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Red Laser (633 nm)
Application Notes

View the video of our collaborator discussing the importance of highly specific monoclonal antibodies for P2RY12 in Microglia research.

Learn more about the validation testing we performed on clone S16007D by watching our Scientific Poster Video.

RRID
AB_2904450 (BioLegend Cat. No. 848013)

Antigen Details

Structure
P2RY12 is a 347 amino acid protein with a molecular mass of 39.8 kD.
Distribution

Tissue distribution: platelets, brain (microglia), lung, appendix, pituitary and adrenal glands.
Cellular distribution: cell membrane and cytosol.

Function
G-protein coupled receptor that inhibits the adenylyl cyclase second messenger system.
Ligand/Receptor
ADP and ATP.
Cell Type
Microglia, Platelets
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
GPCR, Purinergic Receptors
Antigen References

1. Orr AG, et al. 2009. Nat. Neurosci. 12(7):872-8.

Gene ID
70839 View all products for this Gene ID
UniProt
View information about P2RY12 on UniProt.org
Go To Top Version: 1    Revision Date: 11/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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