Biotin anti-human CD366 Tim-3 Antibody anti-CD366

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Clone: F38-2E2 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype: Mouse IgG1, κ
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Product Citations: publications

F38-2E2_Biotin_CD366_Antibody_082118 — PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD4 APC and biotinylated anti-human CD366 (Tim-3, clone F38-2E2, left) or biotinylated mouse IgG1, κ isotype control (right), followed by streptavidin-PE.

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345046

100 µg

203€

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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Tim-3 fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for relevant formats of this clone) include: costimulation¹ (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking^2,3.
RRID: AB_2750217 (BioLegend Cat. No. 345046)

Antigen Details

Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution: Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells
Function: Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Ligand/Receptor: Galectin-9
Cell Type: Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.
Gene ID: 84868 View all products for this Gene ID
UniProt: View information about CD366 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD366 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD366 (Tim-3)	F38-2E2	FC
PE anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 421™ anti-human CD366 (Tim-3)	F38-2E2	FC
Ultra-LEAF™ Purified anti-human CD366 (Tim-3)	F38-2E2	FC,Costim,Block
APC anti-human CD366 (Tim-3)	F38-2E2	FC
PE/Cyanine7 anti-human CD366 (Tim-3)	F38-2E2	FC
PerCP/Cyanine5.5 anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 605™ anti-human CD366 (Tim-3)	F38-2E2	FC
FITC anti-human CD366 (Tim-3)	F38-2E2	FC
Purified anti-human CD366 (Tim-3) (Maxpar® Ready)	F38-2E2	FC,CyTOF®
Brilliant Violet 711™ anti-human CD366 (Tim-3)	F38-2E2	FC
APC/Cyanine7 anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 785™ anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 650™ anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 510™ anti-human CD366 (Tim-3)	F38-2E2	FC
PE/Dazzle™ 594 anti-human CD366 (Tim-3)	F38-2E2	FC
GoInVivo™ Purified anti-human CD366 (Tim-3)	F38-2E2	FC,Costim,Block
APC/Fire™ 750 anti-human CD366 (Tim-3)	F38-2E2	FC
Pacific Blue™ anti-human CD366 (Tim-3)	F38-2E2	FC
Biotin anti-human CD366 (Tim-3)	F38-2E2	FC
TotalSeq™-A0169 anti-human CD366 (Tim-3)	F38-2E2	PG
TotalSeq™-C0169 anti-human CD366 (Tim-3)	F38-2E2	PG
PE/Cyanine5 anti-human CD366 (Tim-3)	F38-2E2	FC
TotalSeq™-B0169 anti-human CD366 (Tim-3)	F38-2E2	PG
Brilliant Violet 750™ anti-human CD366 (Tim-3) Antibody	F38-2E2	FC
TotalSeq™-D0169 anti-human CD366 (Tim-3)	F38-2E2	PG
PE/Fire™ 810 anti-human CD366 (Tim-3)	F38-2E2	FC
PE/Fire™ 640 anti-human CD366 (Tim-3)	F38-2E2	FC
PE/Fire™ 700 anti-human CD366 (Tim-3)	F38-2E2	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 08/22/2018

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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