Biotin anti-human TRA-1-60-R Antibody anti-TRA-1-60-R

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Clone: TRA-1-60-R (See other available formats)
Regulatory Status: RUO
Other Names: Podocalyxin, TRA-1
Isotype: Mouse IgM, κ
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Product Citations: publications

NCCIT (human teratocarcinoma cell line) stained with biotinylated TRA-1-60-R (red line) or biotinylated mouse IgM, κ isotype control (green line) followed by Sav-PE.

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330604

100 µg

231€

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Description

TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R). The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity. Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human embryonal carinoma cell line 2102Ep cl.2A6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, immunohistochemical staining, acetone-fixed frozen sections, immunoprecipitation, and radioimmunoassay.
RRID: AB_1186140 (BioLegend Cat. No. 330604)

Antigen Details

Distribution: Embryonic stemcells
Cell Type: Embryonic Stem Cells
Biology Area: Immunology, Stem Cells
Gene ID: 5420 View all products for this Gene ID
UniProt: View information about TRA-1-60-R on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Stem Cells and Development

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All TRA-1-60-R Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human TRA-1-60-R	TRA-1-60-R	FC,WB,ICC,IP,RIA
Biotin anti-human TRA-1-60-R	TRA-1-60-R	FC
Alexa Fluor® 647 anti-human TRA-1-60-R	TRA-1-60-R	FC
PE anti-human TRA-1-60-R	TRA-1-60-R	FC
Alexa Fluor® 488 anti-human TRA-1-60-R	TRA-1-60-R	FC
PerCP/Cyanine5.5 anti-human TRA-1-60-R	TRA-1-60-R	FC
PE/Cyanine7 anti-human TRA-1-60-R	TRA-1-60-R	FC
TotalSeq™-A1040 anti-human TRA-1-60-R	TRA-1-60-R	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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