Biotin anti-rat CD45 Antibody anti-CD45

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Clone: OX-1 (See other available formats)
Regulatory Status: RUO
Other Names: Leukocyte common antigen, LCA, T200, Ly-5
Isotype: Mouse IgG1, κ
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Product Citations: publications

OX-1_Biotin_021308 — LOU rat splenocytes stained with biotinylated OX-1, followed by Sav-PE

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202203

100 µg

123€

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Description

CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Enriched glycoprotein fraction from Wistar rat thymocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunofluorescence microscopy (acetone fixed, ammonium-thiocyanate separated epidermal sheets)², immunohistochemistry of acetone³-, isopentane-⁴, PLP⁵-, and n-hexane⁶ fixed frozen sections and paraffin-embedded sections^7,8, and partial inhibition of NK cell lysis of syngeneic tumor cell lines¹.
RRID: AB_314004 (BioLegend Cat. No. 202203)

Antigen Details

Structure: Protein tyrosine phosphatase, type I transmembrane protein, 180-220 kD (multiple isoforms and differential glycosylation)
Distribution: All hematopoietic cells except erythrocytes and platelets
Function: T cell receptor and B cell receptor signal transduction
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol.. 15:168.
Gene ID: 24699 View all products for this Gene ID
UniProt: View information about CD45 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD45 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-rat CD45	OX-1	FC,IHC-P,IHC-F,IP
Biotin anti-rat CD45	OX-1	FC
FITC anti-rat CD45	OX-1	FC
PE anti-rat CD45	OX-1	FC
APC/Cyanine7 anti-rat CD45	OX-1	FC
PE/Cyanine7 anti-rat CD45	OX-1	FC
PerCP/Cyanine5.5 anti-rat CD45	OX-1	FC
Alexa Fluor® 488 anti-rat CD45	OX-1	FC
Alexa Fluor® 647 anti-rat CD45	OX-1	FC
Alexa Fluor® 700 anti-rat CD45	OX-1	FC
APC/Fire™ 750 anti-rat CD45	OX-1	FC
Pacific Blue™ anti-rat CD45	OX-1	FC
PE/Dazzle™ 594 anti-rat CD45	OX-1	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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