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Brilliant Violet 421™ anti-human CD25 Antibody

Pricing & Availability
Clone
S20019D (See other available formats)
Regulatory Status
RUO
Other Names
interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. S20019D_BV421_CD25_Antibody_2_080123
Human peripheral blood lymphocytes were stained with anti-human CD25 (clone S20019D) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right). Cells were costained with anti-human CD4 FITC.
  • A. S20019D_BV421_CD25_Antibody_2_080123
    Human peripheral blood lymphocytes were stained with anti-human CD25 (clone S20019D) Brilliant Violet 421™ (left) or mouse IgG1, κ Brilliant Violet 421™ isotype control (right). Cells were costained with anti-human CD4 FITC.
  • B. S20019D_BV421_CD25_Antibody_1_070323
    PHA-activated human peripheral blood lymphocytes were stained with anti-human CD25 (clone S20019D) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
385207 25 tests 148€
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385208 100 tests 300€
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Description

CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD25 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone S20019D can block binding of clone M-A251 on target cells and is blocked by M-A251.

Clone S20019D does not block binding of clones S20019C, BC96, and TÜ69 on target cells.

Clone S20019D does not affect binding of IL-2 to the receptor. 

Clone S20019D shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.

RRID
AB_3068166 (BioLegend Cat. No. 385207)
AB_3068166 (BioLegend Cat. No. 385208)

Antigen Details

Structure
55 kD transmembrane protein
Distribution

Activated T cells, T regulatory cells, monocytes

Function
IL-2 receptor
Interaction
CD122, CD132
Ligand/Receptor
IL-2
Cell Type
Lymphocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Waldmann T. 1991. J Biol Chem. 266:2681-4.
  3. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 07/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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