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Brilliant Violet 650™ anti-mouse CD66a (CEACAM1a) Antibody

Pricing & Availability
Clone
MAb-CC1 (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM1a, Bgp
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
MAb-CC1_BV650_CD66a_Antibody_1_062117
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CD66a (clone Mab-CC1) Brilliant Violet 650™ (top) or mouse IgG1, κ Brilliant Violet 650™ isotype control (bottom).
  • MAb-CC1_BV650_CD66a_Antibody_1_062117
    C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CD66a (clone Mab-CC1) Brilliant Violet 650™ (top) or mouse IgG1, κ Brilliant Violet 650™ isotype control (bottom).
  • MAb-CC1_BV650_CD66a_Antibody_2_062117
Compare all formats See Brilliant Violet 650™ spectral data
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134529 50 µg 229€
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Description

CD66a, known as CEACAM1a, carcinoembryonic antigen-related cell adhesion molecule 1a, is a glycoprotein of the immunoglobulin superfamily and the carcinoembryonic antigen family. Isoforms expressing either two or four alternatively spliced Ig-like domains in mice have been found in a number of epithelial, endothelial, or hematopoietic tissues. CEACAM1a functions as an intercellular adhesion molecule, an angiogenic factor, and a tumor cell growth inhibitor. It also serves as a signal regulatory protein influencing B cell receptor complex-mediated activation. The mouse and human CEACAM1a proteins are targets of viral or bacterial pathogens, respectively. It was reported that targeted disruption of the CEACAM1a gene resulting in a partial ablation of the protein in mice led to reduced susceptibility to virus infection. The antibody recognizes the N-terminal domain of murine CEACAM1a, it does not recognize murine CEACAM1b, an allele in SJL mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL/J mice immunized with deoxycholate extracted proteins from BALB/c intestinal brush border membranes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd. 


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Mab-CC1 clone recognizes the MHV binding domain of CD66a2. Pretreatment of murine fibroblasts with Mab-CC1 prevented binding of MHV-A59 and protected cells from infection with MHV-A592. SJL/J mice are resistant to MHV as they express an alternative allele, clone Mab-CC1 does not react against SJL/J cells2.

Application References

(PubMed link indicates BioLegend citation)
  1. Turner BC, et al. 2004. J. Virol. 78 (10):5486
  2. Williams RK, et al. 1990. J. Virol. 64:3817
  3. Dveksler GS, et al. 1993. Proc. Natl. Acad. Sci. USA. 90:1716
RRID
AB_2687362 (BioLegend Cat. No. 134529)

Antigen Details

Structure
A glycoprotein of the immunoglobulin (Ig) superfamily and the carcinoembryonic antigen family
Distribution

CEACAM1a is abundantly expressed on B cells, granulocytes, some NK cells, monocytes, DCs, and NK cells. It is found on epithelial and endothelial cells.

Function
CEACAM1a is involved in cell adhesion, tumor growth, and viral infection. It regulates on BCR activation of B lymphocytes.
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Granulocytes, Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Nakagaki K, et al. 2005. J. Virol. 79(10):6102
2. Greicius G et al. 2003. J. Leukoc. Biol. 74(1):126
3. Hemmila E et al. 2004. J. Virol. 78(18):10156

Gene ID
26365 View all products for this Gene ID
UniProt
View information about CD66a on UniProt.org
Go To Top Version: 0    Revision Date: 06/21/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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