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Brilliant Violet 650™ anti-mouse I-Ak (Aβk) Antibody

Pricing & Availability
Clone
10-3.6 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Mouse (CWB) IgG2a, κ
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Product Citations
publications
10-36_BV-650_I-Ak_FC_060624
C3H/He mouse splenocytes were stained with anti-mouse CD19 (clone 1D3/CD19) PE and anti-mouse I-Ak (Aβk) Brilliant Violet 650™ (left) or mouse IgG2a, κ Brilliant Violet 650™ isotype control (right).
  • 10-36_BV-650_I-Ak_FC_060624
    C3H/He mouse splenocytes were stained with anti-mouse CD19 (clone 1D3/CD19) PE and anti-mouse I-Ak (Aβk) Brilliant Violet 650™ (left) or mouse IgG2a, κ Brilliant Violet 650™ isotype control (right).
Compare all formats See Brilliant Violet 650™ spectral data
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109917 50 µg 234€
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Description

The 10-3.6 antibody reacts with the β chain of the I-Ak MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-Af,r,s antigens and I-Ag7 of NOD mice; it does not react with other haplotypes (e.g., b, d, p, q).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include immunoprecipitation1,2, protection against autoimmune IDDM3, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth4, and immunohistochemical staining5 of acetone-fixed frozen sections.

This clone does not cross react with other haplotypes (e.g. b, d, p, q).

Application References

(PubMed link indicates BioLegend citation)
  1. Landais D, et al. 1986. J. Immunol. 137:3002. (IP)
  2. Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP)
  3. Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
  4. Alisauskas RM, et al. 1986. Immunopharmacology 12:1.
  5. Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC)
  6. Yui MA, et al. 2010. J. Immunol. 185:284. PubMed
  7. Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)
RRID
AB_3106208 (BioLegend Cat. No. 109917)

Antigen Details

Structure
β chain of MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14960 View all products for this Gene ID
UniProt
View information about I-Ak on UniProt.org
Go To Top Version: 1    Revision Date: 05/23/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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