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Direct-Blot™ HRP anti-E. coli RNA Sigma 70 Antibody

Pricing & Availability
Clone
2G10 (See other available formats)
Regulatory Status
RUO
Other Names
RNA pol sigma 70, rpoD, sigma D, RNA Polymerase
Isotype
Mouse IgG2b
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Product Citations
publications
2G10_DBHRP_Ecoli_RNASigma70_Antibody_092216
15 µg of total protein extract from E. coli was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP (DB-HRP) anti-E. coli RNA Sigma 70 (clone 2G10). Proteins were visualized using chemiluminescence detection.
  • 2G10_DBHRP_Ecoli_RNASigma70_Antibody_092216
    15 µg of total protein extract from E. coli was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP (DB-HRP) anti-E. coli RNA Sigma 70 (clone 2G10). Proteins were visualized using chemiluminescence detection.
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663205 100 µL 272€
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Description

RNA polymerase transcribes DNA to synthesize RNA. Prokaryotes contain a single RNA polymerase- core RNA polymerase. RNA polymerase sigma 70 (also known as RNA pol sigma 70 and rpoD) is a part of a family of sigma factor subunits that are required for specific promoter recognition and initiation. RNA pol sigma 70 is the major housekeeping sigma factor responsible for RNA polymerase recognition out of the majority of promoters necessary to transcribe bacterial genes which are expressed during expansion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Clone 2G10 is extremely useful for identifying the major sigma factor from a variety of bacteria. It does not react with Borellia burgdorferi. Clone 2G10 recognizes epitope 470-486.

Additional reported applications (for the relevant formats) include: functional assay and immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Breyer MJ, et al. 1997. J. Bacterial 179:1404.
  2. Lesley SA, et al. 1989. J. Biol. Chem. 264:3794.
  3. Strickland MS, et al. 1988. Biochemistry 27:5755.
Product Citations
  1. Carobbi A, et al. 2022. Environ Microbiol. 24:4787. PubMed
  2. Hughes KJ, et al. 2020. Cell Rep. 33:108527. PubMed
  3. Piel D, et al. 2020. Environ Microbiol. 4198:22. PubMed
  4. Dar Y, et al. 2022. EMBO Rep. 23:e53981. PubMed
  5. Adams DW, et al. 2019. Nat Microbiol. 1.239583333. PubMed
  6. Yang H, et al. 2021. Cell Discov. 7:90. PubMed
RRID
AB_2629596 (BioLegend Cat. No. 663205)

Antigen Details

Structure
Sigma 70 (σ70) is a 70 kD transcription factor of E. coli RNA polymerase.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
947567 View all products for this Gene ID
UniProt
View information about Sigma 70 on UniProt.org
Go To Top Version: 4    Revision Date: 09/19/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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