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Direct-Blot™ HRP anti-STAT3 Phospho (Tyr705) Antibody

Pricing & Availability
Clone
13A3-1 (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
Isotype
Mouse IgG1, κ
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Product Citations
publications
13A3dash1_DBHRP_STAT3_Tyr705_Antibody_WB_062116
15 µg of total protein extract from HepG2 cells untreated or treated with 10 ng/mL recombinant human IL-6 for 20 minutes was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP anti-STAT3 Phospho (Tyr705) (clone 13A3-1) antibody. The purified antibody was detected using an HRP goat anti-mouse secondary antibody. Proteins were visualized using chemiluminescence detection.
  • 13A3dash1_DBHRP_STAT3_Tyr705_Antibody_WB_062116
    15 µg of total protein extract from HepG2 cells untreated or treated with 10 ng/mL recombinant human IL-6 for 20 minutes was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP anti-STAT3 Phospho (Tyr705) (clone 13A3-1) antibody. The purified antibody was detected using an HRP goat anti-mouse secondary antibody. Proteins were visualized using chemiluminescence detection.
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651011 100 µL 272€
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Description

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated modified synthetic peptide
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.
 

Product Citations
  1. Aung Naing et al. 2018. Cancer cell. 34(5):775-791 . PubMed
RRID
AB_2616951 (BioLegend Cat. No. 651011)

Antigen Details

Structure
STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution

Ubiquitous.

Function
STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.
Cell Sources
Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.
Cell Type
Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References

1. Akira S, et al. 1994. Cell 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145.

Regulation
The small GTPase Rac1 binds and regulates activity of STAT3.
Gene ID
6774 View all products for this Gene ID
UniProt
View information about STAT3 Phospho Tyr705 on UniProt.org
Go To Top Version: 4    Revision Date: 07/27/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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