- Clone
- AA10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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10 µg of total protein extract from HeLa cells was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP anti-Tubulin Beta 3 (TUBB3) (clone AA10). Proteins were visualized using chemiluminescence detection.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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657409 | 100 µL | 272€ |
Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Rat, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - Application Notes
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Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
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Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_2566704 (BioLegend Cat. No. 657409)
Antigen Details
- Structure
- 450 amino acids with predicted molecular weight of 50 kD
- Distribution
-
Cytosol
- Function
- Plays important roles in neuronal cell proliferation and differentiation
- Interaction
- Alpha tubulin, kinesin and dynein
- Cell Type
- Mature Neurons
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
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1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851.
2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015.
3. Locher H, et al. 2013. Differentiation. 85:173.
4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461.
5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726.
6. Koh Y, et al. 2009. Ann. Oncol. 20:1414. - Gene ID
- 10381 View all products for this Gene ID
- UniProt
- View information about Tubulin beta-3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Tubulin β-3 (TUBB3) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Tubulin Beta 3 (TUBB3) | AA10 | WB,ICC |
Alexa Fluor® 488 anti-Tubulin Beta 3 (TUBB3) | AA10 | ICFC,IHC-F,3D IHC |
Alexa Fluor® 647 anti-Tubulin Beta 3 (TUBB3) | AA10 | ICFC,IHC-F,3D IHC,SB |
Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3) | AA10 | IHC-F,ICC,IHC-P,3D IHC |
Direct-Blot™ HRP anti-Tubulin Beta 3 (TUBB3) | AA10 | WB |
Brilliant Violet 421™ anti-Tubulin Beta 3 (TUBB3) | AA10 | IHC-F,ICC,SB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Tubulin Beta 3 (TUBB3)
Mouse brain (lane 1), mouse liver (lane 2), N-tera2 (lane 3)... Whole cell lysates (15 µg protein) from HEL (negative contro... NTERA‐2 cells were fixed with 4% paraformaldehyde (PFA) for ... -
Alexa Fluor® 488 anti-Tubulin Beta 3 (TUBB3)
Human lung adenocarcinoma cell line A549 was treated with Bi... C57BL/6 mouse frozen brain tissue was fixed with 4% paraform... Dissected C57/B6 mouse small intestine was immersed in 4% p... Formalin-fixed, 300 micron-thick mouse kidney section was bl... -
Alexa Fluor® 647 anti-Tubulin Beta 3 (TUBB3)
Human lung adenocarcinoma cell line A549 was treated with Fi... C57BL/6 mouse frozen brain tissue was fixed with 4% paraform... Formalin-fixed, 300 micron-thick mouse large intestine secti... -
Alexa Fluor® 594 anti-Tubulin Beta 3 (TUBB3)
Day-three cultured postnatal C57BL/6 mouse brain cells were ... C57BL/6 mouse frozen brain tissue was fixed with 4% paraform... C57BL/6 mouse frozen brain tissue was fixed with 4% paraform... Human paraffin-embedded cerebellum tissue slices were prepar... Human paraffin-embedded cerebellum tissue slices were prepar... Formalin-fixed, 300 micron-thick mouse brain (cerebellum) se... -
Direct-Blot™ HRP anti-Tubulin Beta 3 (TUBB3)
10 µg of total protein extract from HeLa cells was resolved ... -
Brilliant Violet 421™ anti-Tubulin Beta 3 (TUBB3)
Human lung adenocarcinoma cell line A549 was fixed with 1% p... C57BL/6 mouse frozen brain (cerebellum) tissue was fixed wit...
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