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FITC anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_FITC_061807
Human peripheral blood lymphocytes stained with HIB19 FITC
  • HIB19_FITC_061807
    Human peripheral blood lymphocytes stained with HIB19 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
302205 25 tests 40€
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302206 100 tests 83€
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302256 100 µg 85€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Jiao Q, et al. 2020. Br J Dermatol. 182:648. PubMed
  2. Vaidyanathan S, et al. 2022. Mol Ther. 30:223. PubMed
  3. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  4. Asashima H, et al. 2022. J Clin Invest. 132: . PubMed
  5. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  6. Toepfner N et al. 2018. eLife. 7 pii: e29213. PubMed
  7. Kuri-Magaña H, et al. 2018. Front Immunol. 9:2679. PubMed
  8. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  9. Freund EC, et al. 2023. Methods Mol Biol. 2618:201. PubMed
  10. Aabling RR, et al. 2023. Regen Ther. 23:67. PubMed
  11. Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
  12. Muto M, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016050496. PubMed
  13. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  14. Kerr SC, et al. 2020. Clin Exp Allergy. 50:904. PubMed
  15. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  16. Laustsen A, et al. 2021. Elife. 10:. PubMed
  17. Kaku Y, et al. 2021. Cell Reports. 36(2):109385. PubMed
  18. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  19. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  20. Halim L, et al. 2022. Front Immunol. 13:836549. PubMed
  21. Jama B, Morris G 2014. J Vis Exp. 93: 52257. PubMed
  22. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  23. Lefley D, et al. 2019. Breast Cancer Res. 0.965277778. PubMed
  24. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  25. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  26. Aravena O, et al. 2017. Arthritis Res Ther. 19:8. PubMed
  27. Jing Y, et al. 2021. Signal Transduct Target Ther. 6:345. PubMed
  28. Zhang J, et al. 2022. Nature. 609:369. PubMed
  29. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  30. Mu Z, et al. 2021. Genome Biol. 122:22. PubMed
  31. Wang X, et al. 2010. Haematologica. 95:884. PubMed
  32. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  33. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  34. Schanin J, et al. 2022. Commun Biol. 5:1226. PubMed
  35. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  36. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  37. Lu J, et al. 2021. Front Immunol. 12:732612. PubMed
  38. Cheng Y, et al. 2021. Immunity. 54(8):1825-1840.e7. PubMed
  39. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  40. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  41. LaSalle TJ, et al. 2022. Cell Rep Med. 3:100779. PubMed
  42. Valle-Reyes S, et al. 2020. Cell Physiol Biochem. 54:842. PubMed
  43. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  44. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  45. Motwani MP, et al. 2018. JCI Insight. 3:e94463. PubMed
  46. Bradley D, et al. 2022. Nat Commun. 13:5606. PubMed
  47. Hu Q, et al. 2021. Nat Commun. 12:2186. PubMed
  48. Buters TP, et al. 2021. Br J Clin Pharmacol. Online ahead of print. PubMed
  49. Wang F, et al. 2021. Genomics Proteomics Bioinformatics. 19:208. PubMed
  50. Ruan Y, et al. 2022. Exp Ther Med. 23:47. PubMed
  51. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  52. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  53. Łyszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed
  54. Wells DK, et al. 2020. Cell. 183:818. PubMed
  55. Lareau CA, et al. 2020. Nat Biotechnol. 39:451. PubMed
  56. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  57. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  58. Chen YP, et al. 2020. Cell Res. 30:1024. PubMed
  59. Foote JR, et al. 2019. Front Immunol. 10:188. PubMed
  60. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  61. Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed
  62. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  63. Rusconi M, et al. 2017. Sci Rep. 5.745138889. PubMed
  64. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  65. Lepore M, et al. 2014. J Exp Med. 211:1363. PubMed
RRID
AB_2564143 (BioLegend Cat. No. 302205)
AB_2564143 (BioLegend Cat. No. 302206)
AB_2564143 (BioLegend Cat. No. 302256)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Other Formats

View All CD19 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD19 HIB19 FC
Biotin anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Purified anti-human CD19 HIB19 FC,CyTOF®,IHC-F
APC/Cyanine7 anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
Alexa Fluor® 488 anti-human CD19 HIB19 FC,ICC
Alexa Fluor® 647 anti-human CD19 HIB19 FC,IHC-F
Pacific Blue™ anti-human CD19 HIB19 FC
Alexa Fluor® 700 anti-human CD19 HIB19 FC
PerCP anti-human CD19 HIB19 FC
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Brilliant Violet 421™ anti-human CD19 HIB19 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD19 HIB19 FC
Brilliant Violet 650™ anti-human CD19 HIB19 FC
Brilliant Violet 785™ anti-human CD19 HIB19 FC
Brilliant Violet 510™ anti-human CD19 HIB19 FC
Brilliant Violet 605™ anti-human CD19 HIB19 FC
Brilliant Violet 711™ anti-human CD19 HIB19 FC
Purified anti-human CD19 (Maxpar® Ready) HIB19 FC,CyTOF®
Alexa Fluor® 594 anti-human CD19 HIB19 ICC,IHC-F
PE/Dazzle™ 594 anti-human CD19 HIB19 FC
PE anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
Pacific Blue™ anti-human CD19 HIB19 FC
APC anti-human CD19 HIB19 FC
PE/Cyanine7 anti-human CD19 HIB19 FC
TotalSeq™-A0050 anti-human CD19 HIB19 PG
Brilliant Violet 750™ anti-human CD19 HIB19 FC
TotalSeq™-B0050 anti-human CD19 HIB19 PG
TotalSeq™-C0050 anti-human CD19 HIB19 PG
PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
Spark NIR™ 685 anti-human CD19 HIB19 FC
Ultra-LEAF™ Purified anti-human CD19 HIB19 FC,CyTOF®,Block,IHC
APC/Fire™ 810 anti-human CD19 HIB19 FC
PE/Fire™ 640 anti-human CD19 HIB19 FC
PE/Fire™ 700 anti-human CD19 HIB19 FC
TotalSeq™-D0050 anti-human CD19 HIB19 PG
Spark YG™ 593 anti-human CD19 HIB19 FC
GMP Pacific Blue™ anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
GMP PE anti-human CD19 HIB19 FC
GMP APC anti-human CD19 HIB19 FC
KIRAVIA Blue 520™ anti-human CD19 HIB19 FC
GMP PerCP/Cyanine5.5 anti-human CD19 HIB19 FC
GMP PE/Cyanine7 anti-human CD19 HIB19 FC
Spark Violet™ 500 anti-human CD19 HIB19 FC
PE/Fire™ 810 anti-human CD19 HIB19 FC
Spark Violet™ 423 anti-human CD19 HIB19 FC
Spark UV™ 387 anti-human CD19 HIB19 FC
APC/Fire™ 750 anti-human CD19 HIB19 FC
PE/Cyanine5 anti-human CD19 HIB19 FC
Alexa Fluor® 660 anti-human CD19 HIB19 FC
PerCP/Fire™ 806 anti-human CD19 HIB19 FC
PerCP/Fire™ 780 anti-human CD19 HIB19 FC
Spark PLUS UV395™ anti-human CD19 HIB19 FC
Spark Red™ 718 anti-human CD19 HIB19 FC
FITC anti-human CD19 HIB19 FC
Go To Top Version: 3    Revision Date: 10/03/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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