- Clone
- MEM-59 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Leukosialin, leuokocyte sialoglycoprotein, sialophorin, galactoglyprotein
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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315203 | 25 tests | 67€ | ||||
315204 | 100 tests | 151€ |
CD43 is a 95-135 kD type I transmembrane glycoprotein known as leukosialin, leukocyte sialoglycoprotein, sialophorin, and galactoglyprotein. It is expressed at high levels on the surface of all leukocytes (except on resting B cells) and at low levels on platelets, negative on erythrocytes. The expression of CD43 on T cells and neutrophils is rapidly downregulated by proteolytic shedding after activation with various stimuli. The soluble form of CD43 is present in human serum. It has been reported that CD43 plays a role in anti-adhesive functions, but also mediates adhesion via its interaction with CD54 or some lectins. Similarly, CD43 is implicated in anti-apoptotic and proapoptotic signaling.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human T lymphocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, immunohistochemistry of formalin-fixed paraffin-embedded sections, and T cell activationSup>1,2.
-
Application References
(PubMed link indicates BioLegend citation) -
- Olweus J, et al. 1993. J. Immunol. 151:707.
- Alvarado M, et al. 1995. Eur. J. Immunol. 25:1051.
- Ye L, et al. 2013. Stem Cell Transl Med. 2:558. PubMed
- Product Citations
-
- RRID
-
AB_389246 (BioLegend Cat. No. 315203)
AB_389246 (BioLegend Cat. No. 315204)
Antigen Details
- Structure
- Type I membrane protein, 95-135 kD, soluble form exists in serum
- Distribution
-
Thymocytes, T cells, neutrophils, plasma cells, myelomas
- Function
- Anti-adhesion, mediate adhesion, regulate T cell activation and neutrophil migration, antiapoptotic and proapoptotic signaling
- Interaction
- HIPK2
- Ligand/Receptor
- CD54, Counterreceptor for SN/Siglec-1
- Modification
- Highly glycosylated
- Cell Type
- Thymocytes, T cells, Neutrophils, Plasma cells, B cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Leukocyte Typing IV. Knapp W, et al. (Eds.) Oxford University Press (1989)
2. Stephanova I, et al. 1989. Mol. Immunol. 26:153.
3. Alvarado M, et al. 1995. Eur. J. Immunol. 25:1051.
4. Deckert M, et al. 1992. Eur. J. Immunol. 22:2943
5. Cermak L, et al. 2002. J. Biol. Chem. 227:7955. - Gene ID
- 6693 View all products for this Gene ID
- UniProt
- View information about CD43 on UniProt.org
Other Formats
View All CD43 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
FITC anti-human CD43 | MEM-59 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD43
Human peripheral blood lymphocytes stained with MEM-59 FITC
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