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FITC anti-human CD94 Antibody

Pricing & Availability
Clone
DX22 (See other available formats)
Regulatory Status
RUO
Other Names
KP43
Isotype
Mouse IgG1, κ
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Product Citations
publications
DX22_FITC_070507
Human peripheral blood lymphocytes stained with DX22 FITC
  • DX22_FITC_070507
    Human peripheral blood lymphocytes stained with DX22 FITC
Compare all formats See FITC spectral data
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305504 100 tests 151€
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Description

CD94 is a 43 kD type II transmembrane glycoprotein also known as KP43. CD94 belongs to the C-type lectin superfamily and is present as a covalently linked heterodimer with NKG2 on the cell surface. CD94 is expressed by NK cells, a subset of γδ T cells, and NKT cells. The CD94/NKG2A complex serves as an inhibitory receptor specific for HLA-class I molecules.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation4, inhibition of NK cell-mediated lysis5, immunohistochemical staining of acetone-fixed frozen tissue sections, and spatial biology (IBEX)6,7.

Application References

(PubMed link indicates BioLegend citation)
  1. Mizuki M, et al. 2000. Sarcoidosis Vasc. Diffuse Lung Dis. 17:54.
  2. Phillip J, et al. 1996. Immunity 5:163.
  3. Lazetic S, et al. 1996. J. Immunol. 157:4741.
  4. Lanier LL, et al. 1998. Immunity 8:693.
  5. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  6. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  7. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hu X, et al. 2023. Nat Biotechnol. . PubMed
  2. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
  3. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  4. Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
  5. Wu J, et al. 2021. Front Immunol. 12:601611. PubMed
  6. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  7. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  8. Mallis P, et al. 2020. World J Stem Cells. 12:203. PubMed
  9. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  10. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  11. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  12. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  13. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  14. Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
RRID
AB_314534 (BioLegend Cat. No. 305504)

Antigen Details

Structure
C-type lectin, type II transmembrane glycoprotein, covalently associates with NKG2, 43 kD
Distribution

NK cells , T subset

Function
Inhibits NK function
Cell Type
NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165.
2. Moretta A, et al. 1997. Immunol. Rev. 155:105.

Gene ID
3824 View all products for this Gene ID
UniProt
View information about CD94 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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