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FITC anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_FITC_011409.jpg
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
  • HK1dot4_FITC_011409.jpg
    C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
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128005 50 µg 72€
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128006 500 µg 235€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  2. de la Rosa X, et al. 2018. Am J Pathol. 188:950. PubMed
  3. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  4. Duan Y, et al. 2019. Circ Res. 125:969. PubMed
  5. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  6. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  7. Zaman R, et al. 2021. Immunity. :. PubMed
  8. Liang J, et al. 2021. Cancer Manag Res. 13:6977. PubMed
  9. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  10. Fang C, et al. 2022. J Pathol. 256:310. PubMed
  11. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  12. Du Y, et al. 2022. FASEB J. 36:e22248. PubMed
  13. Helfen A, et al. 2022. Neoplasia. 28:100792. PubMed
  14. Liu X, et al. 2022. Adv Sci (Weinh). 9:e2105650. PubMed
  15. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  16. Campisi L, et al. 2022. Nature. 606:945. PubMed
  17. Silva Pereira S, et al. 2022. Elife. 11: . PubMed
  18. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  19. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  20. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  21. Soni N, et al. 2022. FASEB Bioadv. 4:798. PubMed
  22. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  23. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  24. Meng S, et al. 2023. Stem Cell Investig. 10:2. PubMed
  25. Hettwer J, et al. 2022. Cardiovasc Res. 118:2778. PubMed
  26. Fernández-García V, et al. 2022. Biomed Pharmacother. 148:112769. PubMed
  27. Chen K, et al. 2022. Adv Sci (Weinh). 9:e2104106. PubMed
  28. Mise Y, et al. 2022. Carcinogenesis. 43:647. PubMed
  29. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  30. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  31. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  32. Aso K, et al. 2023. Nat Commun. 14:984. PubMed
  33. Bautista CA, et al. 2023. Front Physiol. 14:1122348. PubMed
  34. He Y, et al. 2023. Biomolecules. 13:. PubMed
  35. Lin CH, et al. 2023. iScience. 26:106904. PubMed
  36. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  37. Keeter WC, et al. 2022. Eur Heart J Open. 2:oeac028. PubMed
  38. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  39. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  40. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  41. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  42. Jones NM, et al. 2019. BMC Cancer. 1.127777778. PubMed
  43. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  44. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  45. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  46. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  47. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  48. You Y, et al. 2016. Sci Rep. 6:20630. PubMed
  49. Dong L, et al. 2021. Cancer Cell. . PubMed
  50. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  51. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  52. Shani O, et al. 2020. Cancer Res. 80:5317. PubMed
  53. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  54. McAlpine CS, et al. 2019. Nature. 566:383. PubMed
  55. Buzas K, et al. 2011. Cytokine. 55:429. PubMed
  56. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  57. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  58. Iwata H, et al. 2016. Nat Commun. 7:12849. PubMed
  59. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  60. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  61. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  62. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  63. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  64. Bhattacharya P, et al. 2022. PLoS Negl Trop Dis. 16:e0010224. PubMed
  65. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  66. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  67. Moreau GB, et al. 2020. Am J Trop Med Hyg. 103:1215. PubMed
  68. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  69. Mirakaj V, et al. 2014. J Exp Med. 211:1037. PubMed
  70. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  71. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  72. Al-Sharea A, et al. 2017. Cell Cycle. 10.1016/j.atherosclerosis.2017.08.010. PubMed
  73. Shin SH, et al. 2022. Front Cell Neurosci. 16:844480. PubMed
  74. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  75. Naler LB, et al. 2022. Commun Biol. 5:102. PubMed
  76. Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed
  77. Xiao P, et al. 2019. J Exp Med. 216:337. PubMed
  78. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  79. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  80. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  81. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  82. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  83. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  84. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  85. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  86. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  87. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  88. Han D, et al. 2021. J Immunol Res. 6688053:2021. PubMed
  89. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  90. Cao Y, et al. 2015. J Biol Chem. 290: 23050-23063. PubMed
  91. Körner A, et al. 2019. Proc Natl Acad Sci U S A. 116:20623. PubMed
  92. Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed
  93. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  94. Körner A,et al. 2017. Cell Death Differ.. 10.1038/cdd.2017.177. PubMed
  95. He J, et al. 2019. J Clin Invest. 130. PubMed
  96. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  97. Xu Y, et al. 2016. Arthritis Rheum. 67: 225-237. PubMed
  98. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  99. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  100. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  101. Monteran L, et al. 2022. Nat Commun. 13:5797. PubMed
  102. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  103. Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed
  104. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  105. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  106. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  107. Tan X, et al. 2015. Sci Rep. 5: 15702. PubMed
  108. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  109. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  110. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  111. Lee S, et al. 2011. J Immunol. 186:6972. PubMed
  112. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
  113. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  114. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  115. Zhao T, et al. 2022. JCI Insight. 7:. PubMed
  116. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  117. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  118. Hou PP, et al. 2020. Molecular Cell. 78(6):1192-1206.e10. PubMed
  119. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  120. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  121. León-Cabrera S, et al. 2014. Int J Biol Sci. 9 948 . PubMed
  122. Alvarez-Breckenridge C, et al. 2012. J Virol. 86:4566. PubMed
  123. Hightower RM, et al. 2019. Mol Ther. 28:189. PubMed
  124. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  125. Chiang N, et al. 2019. J Clin Invest. 129:5294. PubMed
  126. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
  127. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  128. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  129. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  130. Hobbs BE, et al. 2021. Pathogens. 10:. PubMed
  131. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  132. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  133. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  134. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  135. Meng Michelle Xu et al. 2017. Immunity. 47(2):363-373 . PubMed
  136. Caballero S, et al. 2017. Invest Ophthalmol Vis Sci. 58:5164. PubMed
  137. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  138. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  139. Donlan AN, et al. 2021. JCI Insight. 6: . PubMed
  140. Tyagi A, et al. 2021. Nat Commun. 12:474. PubMed
  141. Tang S, et al. 2022. Virol J. 19:32. PubMed
  142. Asahara M, et al. 2022. PLoS One. 17:e0276135. PubMed
  143. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  144. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  145. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  146. Tummala G, et al. 2021. Invest Ophthalmol Vis Sci. 62:41. PubMed
  147. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  148. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  149. Rahtes A, et al. 2020. Front Immunol. 1.484722222. PubMed
  150. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  151. Ngkelo A, et al. 2016. J Exp Med. 213: 1353 - 1374. PubMed
  152. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
  153. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  154. Nguyen HX et al. 2017. J Neurosci. 37(38):9269-9287 . PubMed
  155. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  156. Hegde V, et al. 2015. J Immunol. 194:5211. PubMed
  157. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  158. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  159. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  160. Jones GS, et al. 2020. mSphere. 5:. PubMed
  161. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  162. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
  163. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  164. Reinke S, et al. 2020. Cell Reports. 30(8):2501-2511. PubMed
  165. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  166. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed
RRID
AB_1186134 (BioLegend Cat. No. 128005)
AB_1186134 (BioLegend Cat. No. 128006)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 2    Revision Date: 12/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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