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FITC anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_FITC_011409.jpg
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
  • HK1dot4_FITC_011409.jpg
    C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
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128005 50 µg 72€
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128006 500 µg 235€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Liang J, et al. 2021. Cancer Manag Res. 13:6977. PubMed
  2. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  3. Fang C, et al. 2022. J Pathol. 256:310. PubMed
  4. Xin B, et al. 2022. Hepatology. 76:630. PubMed
  5. Du Y, et al. 2022. FASEB J. 36:e22248. PubMed
  6. Helfen A, et al. 2022. Neoplasia. 28:100792. PubMed
  7. Liu X, et al. 2022. Adv Sci (Weinh). 9:e2105650. PubMed
  8. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  9. Campisi L, et al. 2022. Nature. 606:945. PubMed
  10. Silva Pereira S, et al. 2022. Elife. 11: . PubMed
  11. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  12. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  13. He Y, et al. 2022. Front Immunol. 13:986202. PubMed
  14. Soni N, et al. 2022. FASEB Bioadv. 4:798. PubMed
  15. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
  16. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  17. Meng S, et al. 2023. Stem Cell Investig. 10:2. PubMed
  18. Hettwer J, et al. 2022. Cardiovasc Res. 118:2778. PubMed
  19. Fernández-García V, et al. 2022. Biomed Pharmacother. 148:112769. PubMed
  20. Chen K, et al. 2022. Adv Sci (Weinh). 9:e2104106. PubMed
  21. Mise Y, et al. 2022. Carcinogenesis. 43:647. PubMed
  22. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  23. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  24. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  25. Zaman R, et al. 2021. Immunity. :. PubMed
  26. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  27. de la Rosa X, et al. 2018. Am J Pathol. 188:950. PubMed
  28. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  29. Duan Y, et al. 2019. Circ Res. 125:969. PubMed
  30. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  31. Pagni RL, et al. 2022. Front Immunol. 13:1005937. PubMed
  32. Aso K, et al. 2023. Nat Commun. 14:984. PubMed
  33. Bautista CA, et al. 2023. Front Physiol. 14:1122348. PubMed
  34. He Y, et al. 2023. Biomolecules. 13:. PubMed
  35. Lin CH, et al. 2023. iScience. 26:106904. PubMed
  36. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  37. Keeter WC, et al. 2022. Eur Heart J Open. 2:oeac028. PubMed
  38. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  39. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  40. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  41. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  42. Jones NM, et al. 2019. BMC Cancer. 1.127777778. PubMed
  43. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  44. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  45. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  46. You Y, et al. 2016. Sci Rep. 6:20630. PubMed
  47. Dong L, et al. 2021. Cancer Cell. . PubMed
  48. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  49. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  50. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  51. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  52. Shani O, et al. 2020. Cancer Res. 80:5317. PubMed
  53. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  54. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  55. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  56. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  57. Buzas K, et al. 2011. Cytokine. 55:429. PubMed
  58. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  59. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  60. Iwata H, et al. 2016. Nat Commun. 7:12849. PubMed
  61. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  62. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  63. McAlpine CS, et al. 2019. Nature. 566:383. PubMed
  64. Bhattacharya P, et al. 2022. PLoS Negl Trop Dis. 16:e0010224. PubMed
  65. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  66. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  67. Moreau GB, et al. 2020. Am J Trop Med Hyg. 103:1215. PubMed
  68. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  69. Mirakaj V, et al. 2014. J Exp Med. 211:1037. PubMed
  70. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  71. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  72. Al-Sharea A, et al. 2017. Cell Cycle. 10.1016/j.atherosclerosis.2017.08.010. PubMed
  73. Shin SH, et al. 2022. Front Cell Neurosci. 16:844480. PubMed
  74. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  75. Naler LB, et al. 2022. Commun Biol. 5:102. PubMed
  76. Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed
  77. Xiao P, et al. 2019. J Exp Med. 216:337. PubMed
  78. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  79. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  80. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  81. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  82. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  83. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  84. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  85. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  86. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  87. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  88. Han D, et al. 2021. J Immunol Res. 6688053:2021. PubMed
  89. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  90. Cao Y, et al. 2015. J Biol Chem. 290: 23050-23063. PubMed
  91. Körner A, et al. 2019. Proc Natl Acad Sci U S A. 116:20623. PubMed
  92. Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed
  93. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  94. Monteran L, et al. 2022. Nat Commun. 13:5797. PubMed
  95. Xu Y, et al. 2016. Arthritis Rheum. 67: 225-237. PubMed
  96. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  97. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  98. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  99. Körner A,et al. 2017. Cell Death Differ.. 10.1038/cdd.2017.177. PubMed
  100. He J, et al. 2019. J Clin Invest. 130. PubMed
  101. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  102. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  103. Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed
  104. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  105. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  106. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  107. Tan X, et al. 2015. Sci Rep. 5: 15702. PubMed
  108. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  109. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  110. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  111. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  112. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  113. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
  114. Lee S, et al. 2011. J Immunol. 186:6972. PubMed
  115. Zhao T, et al. 2022. JCI Insight. 7:. PubMed
  116. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  117. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  118. Hou PP, et al. 2020. Molecular Cell. 78(6):1192-1206.e10. PubMed
  119. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  120. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  121. León-Cabrera S, et al. 2014. Int J Biol Sci. 9 948 . PubMed
  122. Alvarez-Breckenridge C, et al. 2012. J Virol. 86:4566. PubMed
  123. Hightower RM, et al. 2019. Mol Ther. 28:189. PubMed
  124. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  125. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  126. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
  127. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  128. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  129. Chiang N, et al. 2019. J Clin Invest. 129:5294. PubMed
  130. Hobbs BE, et al. 2021. Pathogens. 10:. PubMed
  131. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  132. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  133. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  134. Donlan AN, et al. 2021. JCI Insight. 6: . PubMed
  135. Tyagi A, et al. 2021. Nat Commun. 12:474. PubMed
  136. Tang S, et al. 2022. Virol J. 19:32. PubMed
  137. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  138. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  139. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  140. Meng Michelle Xu et al. 2017. Immunity. 47(2):363-373 . PubMed
  141. Caballero S, et al. 2017. Invest Ophthalmol Vis Sci. 58:5164. PubMed
  142. Asahara M, et al. 2022. PLoS One. 17:e0276135. PubMed
  143. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  144. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  145. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  146. Tummala G, et al. 2021. Invest Ophthalmol Vis Sci. 62:41. PubMed
  147. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  148. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  149. Rahtes A, et al. 2020. Front Immunol. 1.484722222. PubMed
  150. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  151. Ngkelo A, et al. 2016. J Exp Med. 213: 1353 - 1374. PubMed
  152. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
  153. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  154. Nguyen HX et al. 2017. J Neurosci. 37(38):9269-9287 . PubMed
  155. Jones GS, et al. 2020. mSphere. 5:. PubMed
  156. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  157. Hegde V, et al. 2015. J Immunol. 194:5211. PubMed
  158. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  159. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  160. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  161. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  162. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
  163. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  164. Reinke S, et al. 2020. Cell Reports. 30(8):2501-2511. PubMed
  165. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  166. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed
RRID
AB_1186134 (BioLegend Cat. No. 128005)
AB_1186134 (BioLegend Cat. No. 128006)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 2    Revision Date: 12/09/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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